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Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Layered materials (LMs) are at the centre of an ever increasing research effort due to their potential use in a variety of applications. The presence of imperfections, such as bi- or multilayer areas, holes, grain boundaries, isotropic and…

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

Light sheet fluorescence microscopy is able to image large specimen with high resolution by imaging the sam- ples from multiple angles. Multi-view deconvolution can significantly improve the resolution and contrast of the images, but its…

Quantitative Methods · Quantitative Biology 2014-12-03 Stephan Preibisch , Fernando Amat , Evangelia Stamataki , Mihail Sarov , Robert H. Singer , Eugene Myers , Pavel Tomancak

We introduce the light-sheet confocal quantum diamond microscope (LC-QDM) for widefield 3D quantum sensing with efficient confocal readout. The LC-QDM leverages light-sheet illumination and laser scanning confocal methods to enable…

Quantum Physics · Physics 2025-03-04 Daniel G. Ang , Jiashen Tang , Ronald L. Walsworth

SDF-based differential rendering frameworks have achieved state-of-the-art multiview 3D shape reconstruction. In this work, we re-examine this family of approaches by minimally reformulating its core appearance model in a way that…

Computer Vision and Pattern Recognition · Computer Science 2025-03-26 Briac Toussaint , Diego Thomas , Jean-Sébastien Franco

Reconstituted cytoskeleton composites have emerged as a valuable model system for studying non equilibrium soft matter. Faithful capture of the dynamics of these three dimensional, dense networks calls for optical sectioning often…

Instrumentation and Detectors · Physics 2023-05-01 Nathan Felcher , Daisy Achiriloaie , Brian Lee , Ryan McGorty , Janet Sheung

Lensless imaging is an important and challenging problem. One notable solution to lensless imaging is a single pixel camera which benefits from ideas central to compressive sampling. However, traditional single pixel cameras require many…

Computer Vision and Pattern Recognition · Computer Science 2017-03-31 Guy Satat , Matthew Tancik , Ramesh Raskar

In spite of major advances in both ground- and space-based instrumentation, the ultra-low-surface brightness universe (ULSB) still remains a largely unexplored volume in observational parameter space. ULSB observations provide unique…

Instrumentation and Methods for Astrophysics · Physics 2019-08-07 Simona Lombardo , Eduard Muslimov , Gerard Lemaître , Emmanuel Hugot

We present a novel deep learning approach to reconstruct confocal microscopy stacks from single light field images. To perform the reconstruction, we introduce the LFMNet, a novel neural network architecture inspired by the U-Net design. It…

Image and Video Processing · Electrical Eng. & Systems 2020-03-25 Josue Page , Federico Saltarin , Yury Belyaev , Ruth Lyck , Paolo Favaro

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

Confocal microscopy is the cornerstone of cellular biology and biomedical research due to its non-destructive imaging, compatibility with live cells, sensitivity, optical sectioning, and subcellular resolution. To meet the demand for rapid…

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

The main objective of the Wide Field Monitor (WFM) on the LOFT mission is to provide unambiguous detection of the high-energy sources in a large field of view, in order to support science operations of the LOFT primary instrument, the LAD.…

Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz),…

Ultrasound-switchable fluorescence (USF) imaging was recently developed to overcome the limitation of the poor spatial resolution of the conventional fluorescence imaging in centimeter-deep tissue. However, in vivo USF imaging has not been…

Biological Physics · Physics 2018-10-12 Tingfeng Yao , Shuai Yu , Yang Liu , Baohong Yuan

We report a light-field based method that allows the optical encryption of three-dimensional (3D) volumetric information at the microscopic scale in a single 2D light-field image. The system consists of a microlens array and an array of…

Cryptography and Security · Computer Science 2016-10-28 Haoyu Li , Changliang Guo , Inbarasan Muniraj , Bryce C. Schroeder , John T. Sheridan , Shu Jia

Dual-comb microscopy (DCM), based on a combination of dual-comb spectroscopy (DCS) with two-dimensional spectral encoding (2D-SE), is a promising method for scan-less confocal laser microscopy giving an amplitude and phase image contrast…

In image-guided liver surgery, 3D-3D non-rigid registration methods play a crucial role in estimating the mapping between the preoperative model and the intraoperative surface represented as point clouds, addressing the challenge of tissue…

Image and Video Processing · Electrical Eng. & Systems 2025-03-18 Zixin Yang , Richard Simon , Kelly Merrell , Cristian. A. Linte