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As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and…

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

Thick biological tissues give rise to not only the scattering of incoming light waves, but also aberrations of the remaining unscattered waves. Due to the inability of existing optical imaging methodologies to overcome both of these…

This is the third article in a series of three dealing with the exploitation of speckle for imaging purposes. In complex media, a fundamental limit is the multiple scattering phenomenon that completely blurs the imaging process in depth.…

Applied Physics · Physics 2026-02-06 Elsa Giraudat , Flavien Bureau , William Lambert , Mathias Fink , Alexandre Aubry

In optical imaging, light propagation is affected by the inhomogeneities of the medium. Sample-induced aberrations and multiple scattering can strongly degrade the image resolution and contrast. Based on a dynamic correction of the incident…

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

Deep-tissue optical imaging suffers from the reduction of resolving power due to tissue-induced optical aberrations and multiple scattering noise. Reflection matrix approaches recording the maps of backscattered waves for all the possible…

Optical-resolution fluorescence imaging through and within complex samples presents a significant challenge due to random light scattering, with substantial implications across multiple fields. While significant advancements in coherent…

Optics · Physics 2023-12-27 Gil Weinberg , Elad Sunray , Ori Katz

This is the first article in a series of two dealing with a matrix approach for aberration quantification and correction in ultrasound imaging. Advanced synthetic beamforming relies on a double focusing operation at transmission and…

Applied Physics · Physics 2022-12-06 William Lambert , Justine Robin , Laura A. Cobus , Mathias Fink , Alexandre Aubry

Sensing and manipulating targets hidden under scattering media are universal problems that take place in applications ranging from deep-tissue optical imaging to laser surgery. A major issue in these applications is the shallow light…

Imaging through complex scattering media is severely limited by aberrations and scattering which obscure images and reduce resolution. Confocal and temporal gatings partly filter out multiple scattering but are severely degraded by…

Optics · Physics 2026-05-18 Yiwen Zhang , Minh Dinh , Zeyu Wang , Tianhao Zhang , Tianhang Chen , Chia Wei Hsu

Matrix imaging paves the way towards a next revolution in wave physics. Based on the response matrix recorded between a set of sensors, it enables an optimized compensation of aberration phenomena and multiple scattering events that usually…

Image and Video Processing · Electrical Eng. & Systems 2023-11-03 Flavien Bureau , Justine Robin , Arthur Le Ber , William Lambert , Mathias Fink , Alexandre Aubry

We present a laser scanning reflection-matrix microscopy combining the scanning of laser focus and the wide-field mapping of the electric field of the backscattered waves for eliminating higher-order aberrations even in the presence of…

Biological Physics · Physics 2019-10-11 Seokchan Yoon , Hojun Lee , Jin Hee Hong , Yong-Sik Lim , Wonshik Choi

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

Optical spectroscopy is a fundamental tool in numerous areas of science and technology. Much effort has focused on miniaturizing spectrometers, but thus far at the cost of high spectral resolution and broad operating range. Here, we…

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

We introduce a novel reflection-mode diffraction tomography technique that enables simultaneous recovery of forward and backward scattering information for high-resolution 3D refractive index reconstruction. Our technique works by imaging a…

Optics · Physics 2025-02-12 Tongyu Li , Jiabei Zhu , Yi Shen , Lei Tian

Focusing waves inside inhomogeneous media is a fundamental problem for imaging. Spatial variations of wave velocity can strongly distort propagating wavefronts and degrade image quality. Adaptive focusing can compensate for such aberration,…

Image and Video Processing · Electrical Eng. & Systems 2020-06-15 William Lambert , Laura A. Cobus , Thomas Frappart , Mathias Fink , Alexandre Aubry

Three-dimensional phase contrast imaging of multiply-scattering samples in X-ray and electron microscopy is extremely challenging, due to small numerical apertures, the unavailability of wavefront shaping optics, and the highly nonlinear…

Computational Physics · Physics 2021-06-02 Philipp M Pelz , Hamish G Brown , Jim Ciston , Scott D Findlay , Yaqian Zhang , Mary Scott , Colin Ophus
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