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The folding dynamics of proteins at the single molecule level has been studied with single-molecule force spectroscopy (SMFS) experiments for twenty years, but a common standardized method for the analysis of the collected data and for the…

Biomolecules · Quantitative Biology 2018-09-28 Nicola Galvanetto , Andrea Perissinotto , Andrea Pedroni , Vincent Torre

Single-particle tracking allows to infer the motion of single molecules in living cells. When we observe a long trajectory (more than 100 points), it is possible that the particle switches mode of motion over time. Then, fitting a single…

Methodology · Statistics 2018-04-16 Vincent Briane , Charles Kervrann , Myriam Vimond

A computationally-efficient method for evaluating friction in molecular rotary bearings is presented. This method estimates drag from fluctuations in molecular dynamics simulations via the fluctuation-dissipation theorem. This is effective…

Soft Condensed Matter · Physics 2017-08-09 Tad Hogg , Matthew S. Moses , Damian G. Allis

The prediction of the three-dimensional native structure of proteins from the knowledge of their amino acid sequence, known as the protein folding problem, is one of the most important yet unsolved issues of modern science. Since the…

Biological Physics · Physics 2008-11-24 Pablo Echenique

We present a novel statistical mechanics formalism for the theoretical description of the process of protein folding$\leftrightarrow$unfolding transition in water environment. The formalism is based on the construction of the partition…

Biological Physics · Physics 2010-05-20 A. V. Yakubovich , A. V. Solov'yov , W. Greiner

We present a novel Monte Carlo simulation of protein folding, in which all heavy atoms are represented as interacting hard spheres. This model includes all degrees of freedom relevant to folding - all sidechain and backbone torsions - and…

Soft Condensed Matter · Physics 2007-05-23 J. Shimada , E. L. Kussell , E. I. Shakhnovich

We study the impact of mutations (changes in amino acid sequence) on the thermodynamics of simple protein-like heteropolymers consisting of N monomers, representing the amino acid sequence. The sequence is designed to fold into its native…

Condensed Matter · Physics 2009-10-30 G. Tiana , R. A. Broglia , H. E. Roman , E. Vigezzi , E. Shakhnovich

Living systems operate far from thermodynamic equilibrium. Enzymatic activity can induce broken detailed balance at the molecular scale. This molecular scale breaking of detailed balance is crucial to achieve biological functions such as…

Biological Physics · Physics 2018-05-02 F. Gnesotto , F. Mura , J. Gladrow , C. P. Broedersz

Atomically detailed simulations of RNA folding have proven very challenging in view of the difficulties of developing realistic force fields and the intrinsic computational complexity of sampling rare conformational transitions. To tackle…

Soft Condensed Matter · Physics 2022-05-26 Gianmarco Lazzeri , Cristian Micheletti , Samuela Pasquali , Pietro Faccioli

With the help of force spectroscopy, several analytical theories aim at estimating the rate coefficient of folding for various proteins. Nevertheless, a chief bottleneck lies in the fact that there is still no perfect consensus on how does…

Soft Condensed Matter · Physics 2020-04-30 Aviel Chaimovich , Christian Leitold , Christoph Dellago

In the cell, proteins fold and perform complex functions through global structural rearrangements. Function requires a protein to be at the brink of stability to be susceptible to small environmental fluctuations, yet stable enough to…

Proteins fold to a specific functional conformation with a densely packed hydrophobic core that controls their stability. We develop a geometric, yet all-atom model for proteins that explains the universal core packing fraction of…

Soft Condensed Matter · Physics 2025-03-28 Alex T. Grigas , Zhuoyi Liu , Jack A. Logan , Mark D. Shattuck , Corey S. O'Hern

Conformational dynamics is crucial for ribonucleic acid (RNA) function. Techniques such as nuclear magnetic resonance, cryo-electron microscopy, small- and wide-angle X-ray scattering, chemical probing, single-molecule F\"orster resonance…

Biomolecules · Quantitative Biology 2025-01-31 Mattia Bernetti , Giovanni Bussi

We investigate the folding behavior of protein sequences by numerically studying all sequences with maximally compact lattice model through exhaustive enumeration. We get the prion-like behavior of protein folding. Individual proteins…

Biomolecules · Quantitative Biology 2014-11-18 Yong-Yun Ji , You-Quan Li , Jun-Wen Mao , Xiao-Wei Tang

The escape process from the native valley for proteins subjected to a constant stretching force is examined using a model for a Beta-barrel. For a wide range of forces, the unfolding dynamics can be treated as one-dimensional diffusion,…

Statistical Mechanics · Physics 2012-01-18 Stefano Luccioli , Alberto Imparato , Simon Mitternacht , Anders Irbaeck , Alessandro Torcini

The computer-aided folding of biomolecules, particularly RNAs, is one of the most difficult challenges in computational structural biology. RNA tetraloops are fundamental RNA motifs playing key roles in RNA folding and RNA-RNA and…

Biomolecules · Quantitative Biology 2016-09-28 Petra Kührová , Robert B. Best , Sandro Bottaro , Giovanni Bussi , Jiří Šponer , Michal Otyepka , Pavel Banáš

We study the driven Brownian motion of hard rods in a one-dimensional cosine potential with an amplitude large compared to the thermal energy. In a closed system, we find surprising features of the steady-state current in dependence of the…

Statistical Mechanics · Physics 2018-10-24 Dominik Lips , Artem Ryabov , Philipp Maass

Using a structure-based coarse-grained model of proteins, we study the mechanism of unfolding of knotted proteins through heating. We find that the dominant mechanisms of unfolding depend on the temperature applied and are generally…

Biomolecules · Quantitative Biology 2017-01-23 Yani Zhao , Mateusz Chwastyk , Marek Cieplak

Folding properties of a two-dimensional toy protein model containing only two amino-acid types, hydrophobic and hydrophilic, respectively, are analyzed. An efficient Monte Carlo procedure is employed to ensure that the ground states are…

chem-ph · Physics 2016-08-31 Anders Irbäck , Carsten Peterson , Frank Potthast

Many aspects of the study of protein folding and dynamics have been affected by the recent advances in machine learning. Methods for the prediction of protein structures from their sequences are now heavily based on machine learning tools.…

Biological Physics · Physics 2019-11-25 Frank Noé , Gianni De Fabritiis , Cecilia Clementi