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Protein folding is an indispensable process for the majority of proteins after their synthesis from ribosomes in the cell. Most in vitro protein folding studies have focused on single-domain proteins. Hence, it is important to understand…

Biological Physics · Physics 2022-02-08 Ganesh Agam , Anders Barth , Don C. Lamb

The atomistic characterization of the transition state is a fundamental step to improve the understanding of the folding mechanism and the function of proteins. From a computational point of view, the identification of the conformations…

Biomolecules · Quantitative Biology 2015-06-05 Guido Tiana , Carlo Camilloni

Small mechanical forces play important functional roles in many crucial cellular processes, including in the dynamical behavior of the cytoskeleton and in the regulation of osmotic pressure through membrane-bound proteins. Molecular…

Chemical Physics · Physics 2023-10-20 Yuvraj Singh , Glen M. Hocky

Inspired by its central role in many biological processes, the transport of biopolymers across nanoscale pores is at the heart of a single-molecule sensing technology aimed at nucleic acid and protein sequencing, as well as biomarker…

Biological Physics · Physics 2025-10-16 Martin Charron , Breeana Elliott , Nada Kerrouri , Liqun He , Vincent Tabard-Cossa

We derive exact solutions of simplified models for the temporal evolution of the protein concentration within a cell population arbitrarily far from the stationary state. We show that monitoring the dynamics can assist in modeling and…

Biomolecules · Quantitative Biology 2015-05-13 Sandro Azaele , Jayanth R. Banavar , Amos Maritan

The role of thermal pressure fluctuation excited within tightly packaged DNA prior to ejection from protein capsid shells is discussed in a model calculation. At equilibrium before ejection we assume the DNA is folded many times into a…

Biological Physics · Physics 2010-12-22 Michael J. Harrison

Thermodynamic bulk measurements of binding reactions critically rely on the validity of the law of mass action and the assumption of a dilute solution. Yet important biological systems such as allosteric ligand-receptor binding,…

Biological Physics · Physics 2017-03-31 Joan Camunas-Soler , Anna Alemany , Felix Ritort

Confined motions in complex environments are ubiquitous in microbiology. These situations invariably involve the intricate coupling between fluid flow, soft boundaries, surface forces and fluctuations. In the present study, such a coupling…

Soft Condensed Matter · Physics 2024-05-24 Nicolas Fares , Maxime Lavaud , Zaicheng Zhang , Aditya Jha , Yacine Amarouchene , Thomas Salez

Protein folding produces characteristic and functional three-dimensional structures from unfolded polypeptides or disordered coils. The emergence of extraordinary complexity in the protein folding process poses astonishing challenges to…

Biomolecules · Quantitative Biology 2013-08-14 Kelin Xia , Guo-Wei Wei

Torsional-space Monte Carlo simulations of flexible molecules are usually based on the assumption that all values of dihedral angles have equal probability in the absence of atomic interactions. In the present paper it is shown that this…

Chemical Physics · Physics 2007-05-23 Andreas Kraemer

The denaturation of double-stranded DNA as function of force and temperature is discussed. At room temperature, sequence heterogeneity dominates the physics of single molecule force-extension curves starting about 7 piconewtons of below a…

Condensed Matter · Physics 2007-05-23 David R. Nelson

Experimental variables of optical tweezers instrumentation that affect RNA folding/unfolding kinetics were investigated. A model RNA hairpin, P5ab, was attached to two micron-sized beads through hybrid RNA/DNA handles; one bead was trapped…

Biological Physics · Physics 2009-11-13 J. -D. Wen , M. Manosas , P. T. X. Li , S. B. Smith , C. Bustamante , F. Ritort , I. Tinoco

The statistical mechanics of heteropolymer structure formation is studied in the context of RNA secondary structures. A designed RNA sequence biased energetically towards a particular native structure (a hairpin) is used to study the…

Statistical Mechanics · Physics 2009-10-31 R. Bundschuh , T. Hwa

A set of interacting vortices in $2D$ in the presence of a substrate with square symmetry and at filling ratio $1$ can display a low temperature solid phase where only one of the reciprocal lattice vectors of the substrate is…

Soft Condensed Matter · Physics 2020-08-14 Toby Joseph

The nanomechanical response of a folded single protein, the natural nanomachine responsible for myriad biological processes, provides insight into its function. The conformational flexibility of a folded state, characterized by its…

Biological Physics · Physics 2022-10-13 Surya Pratap S Deopa , Shatruhan Singh Rajput , Aadarsh Kumar , Shivprasad Patil

We explain the physical basis of a model for small globular proteins with water interactions. The water is supposed to access the protein interior in an "all-or-none" manner during the unfolding of the protein chain. As a consequence of…

Condensed Matter · Physics 2009-10-31 Audun Bakk , Alex Hansen , Kim Sneppen

The goal of this PhD thesis was to characterize the properties of friction in nanotubes and from a more general point of view the understanding of the microscopic origin of friction. Indeed, the relative simplicity of the system allows us…

Mesoscale and Nanoscale Physics · Physics 2009-11-11 J. Servantie

While Kramers' rates have been studied for almost a century, the transition path time between states has only recently received attention. Transition paths between different energy levels are expected to be indistinguishable in shape and…

Statistical Mechanics · Physics 2020-06-02 Jannes Gladrow , Marco Ribezzi-Crivellari , Felix Ritort , Ulrich F. Keyser

Self Consistent Normal Mode Analysis (SCNMA) is applied to heme c type cytochrome f to study temperature dependent protein motion. Classical Normal Mode Analysis (NMA) assumes harmonic behavior and the protein Mean Square Displacement (MSD)…

Biological Physics · Physics 2013-05-29 Jianguang Guo , Timo Budarz , Joshua M. Ward , Earl W. Prohofsky

We use a free energy functional theory to elucidate general properties of heterogeneously ordering, fast folding proteins, and we test our conclusions with lattice simulations. We find that both structural and energetic heterogeneity can…

Disordered Systems and Neural Networks · Physics 2009-10-31 Steven S. Plotkin , Jose N. Onuchic