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Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

Optics · Physics 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian

Interferometric imaging is a well established method to image phase objects by mixing the image wavefront with a reference one on a CCD camera. It has also been applied to fast transient phenomena, mostly through the analysis of single…

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

A recently introduced two-channel confocal microscope with correlated detection promises up to 50% improvement in transverse spatial resolution [Simon, Sergienko, Optics Express {\bf 18}, 9765 (2010)] via the use of photon correlations.…

Quantum Physics · Physics 2015-05-18 D. S. Simon , A. V. Sergienko

We present a scalable scheme to achieve photon distillation, i.e. the preparation of a single photon with reduced indistinguishability error out of multiple imperfect photons, based on multiphoton interference in Fourier matrices. Our…

Quantum Physics · Physics 2025-04-04 F. H. B. Somhorst , B. K. Sauër , S. N. van den Hoven , J. J. Renema

Colocalization is a powerful tool to study the interactions between fluorescently labeled molecules in biological fluorescence microscopy. However, existing techniques for colocalization analysis have not undergone continued development…

Methodology · Statistics 2018-02-14 Shulei Wang , Ellen T. Arena , Kevin W. Eliceiri , Ming Yuan

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Modeling these features is ever more important in quantitatively interpreting microscopy images collected at scales on par or…

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of…

Determining the number of fluorescent entities that are coupled to a given molecule (DNA, protein, etc.) is a key point of numerous biological studies, especially those based on a single molecule approach. Reliable methods are important, in…

Fundamental understanding of biological pathways requires minimally invasive nanoscopic optical resolution imaging. Many approaches to high-resolution imaging rely on localization of single emitters, such as fluorescent molecule or quantum…

Quantum Physics · Physics 2022-04-05 Shuo Li , Wenchao Li , Vladislav V. Yakovlev , Allison Kealy , Andrew D. Greentree

The measurement problem is approached from a mechanical aspect, utilizing schematics to better assist in visualizing the boundary and the interplay between the quantum and classical worlds. This approach graphically illustrates what happens…

General Physics · Physics 2009-09-09 Fred H. Thaheld

One of the main limitations for the resolution of optical instruments is the size of the sensor's pixels. In this paper we introduce a new sub pixel resolution algorithm to enhance the resolution of images. This method is based on the…

Instrumentation and Detectors · Physics 2012-11-12 Siamak Khademi , Ahmad Darudi , Zahra Abbasi

A novel method to estimate the pixels simultaneous detection probability and the spatial resolution of pixelized detectors is proposed, which is based on the determination of the statistical correlations between detector neighbor pixels.…

Medical Physics · Physics 2008-02-25 V. Grabski

Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically-transparent specimens such as biological samples, but lacks chemical sensitivity limiting its application to…

Optics · Physics 2019-09-04 Miu Tamamitsu , Keiichiro Toda , Ryoichi Horisaki , Takuro Ideguchi

This work studies the problem of few-shot object counting, which counts the number of exemplar objects (i.e., described by one or several support images) occurring in the query image. The major challenge lies in that the target objects can…

Computer Vision and Pattern Recognition · Computer Science 2022-09-13 Zhiyuan You , Kai Yang , Wenhan Luo , Xin Lu , Lei Cui , Xinyi Le

This paper presents a renewed overview of photosensor oculography (PSOG), an eye-tracking technique based on the principle of using simple photosensors to measure the amount of reflected (usually infrared) light when the eye rotates.…

Computer Vision and Pattern Recognition · Computer Science 2017-07-20 Ioannis Rigas , Hayes Raffle , Oleg V. Komogortsev

Polarization is a fundamental property of light that carries distinct and valuable information. Consequently, its precise measurement is crucial for numerous applications, including biomedical imaging, remote sensing, and optical…

We experimentally demonstrate a testing strategy for boson samplers that is based on efficiently computable expressions for the output photon counting distributions binned over multiple optical modes. We apply this method to validate boson…

Microscopy is the workhorse of the physical and life sciences, producing crisp images of everything from atoms to cells well beyond the capabilities of the human eye. However, the analysis of these images is frequently little better than…

Soft Condensed Matter · Physics 2017-10-18 Matthew Bierbaum , Brian D. Leahy , Alexander A. Alemi , Itai Cohen , James P. Sethna

The learning of the physical world relies on sensing and data post-processing. When the signals are weak, multidimensional and correlated, the performance of learning is often bottlenecked by the quality of sensors, calling for integrating…

Quantum Physics · Physics 2025-12-09 Jia-Jin Feng , Anthony J. Brady , Quntao Zhuang