Fluorescence Microscopy: a statistics-optics perspective
Optics
2023-10-19 v3 Biological Physics
Data Analysis, Statistics and Probability
Abstract
Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Modeling these features is ever more important in quantitatively interpreting microscopy images collected at scales on par or smaller than light's wavelength. Here we review the optics responsible for generating fluorescent images, fluorophore properties, microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement.
Cite
@article{arxiv.2304.01456,
title = {Fluorescence Microscopy: a statistics-optics perspective},
author = {Mohamadreza Fazel and Kristin S. Grussmayer and Boris Ferdman and Aleksandra Radenovic and Yoav Shechtman and Jörg Enderlein and Steve Pressé},
journal= {arXiv preprint arXiv:2304.01456},
year = {2023}
}