Related papers: Fluorescence Microscopy: a statistics-optics persp…
Based on diffraction theory and the propagation of the light, Fourier optics is a powerful tool allowing the estimation of a visible-range imaging system to transfer the spatial frequency components of an object. The analyses of the imaging…
Optical spectroscopy is an important and widely used technique, for instance, to characterize new materials and to identify unknown compounds. Spectra are typically reported as a function of the wavelength of light, yet the information…
Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…
With the proliferation of inexpensive CMOS cameras, medical imaging experiences a noticeable influx of new technologies. While anatomical imaging is based on well-established principles of photography, physiological optical imaging is a…
The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…
Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…
We present a new, fully generative model of optical telescope image sets, along with a variational procedure for inference. Each pixel intensity is treated as a Poisson random variable, with a rate parameter dependent on latent properties…
Fluorescence spectroscopy and modeling provide powerful means to characterize biomacromolecular structures, dynamics, and interactions. F\"orster resonance energy transfer serves as a key technique for this due to its nanometer-scale…
Recently, light-assisted nanofabrication have been introduced, such as the synthesis of quantum dots using photo-induced desorption that yields reduced size fluctuations, or metal sputtering under light illumination resulting in…
Our work aims at using quantitative imaging tools to complement the limitation of noise encountered by high resolution fluorescence microscopy methods. Several cycles of fluorophore activation, imaging and deactivation produce a sequence of…
We extend entangled coincidence (ghost) imaging to fluorescent samples. Given the entangled photon correlation, one photon of the pair carries information on where the other photon has been absorbed and has produced fluorescence in a…
The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…
Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…
The micro-arcsecond scale structure of the seemingly point-like images in lensed quasars, though unobservable, is nevertheless much studied theoretically, because it affects the observable (or macro) brightness, and through that provides…
Chromatic effects are usually associated with refractive optics, so reflective telescopes are assumed to be free from them. We show that all-reflective optics still bears significant levels of such perturbations, which is especially…
The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…
The nature of light sources is defined by the statistical fluctuations of the electromagnetic field. As such, the photon statistics of light sources are typically associated with distinct emitters. Here, we demonstrate the possibility of…
A comprehensive new approach is presented for deriving probability densities of physical properties characterizing lens or source that constitute an observed galactic microlensing event. While previously encountered problems are overcome,…
Microwave, submillimetre-wave, and far-infrared phased arrays are of considerable importance for astronomy. We consider the behaviour imaging phased arrays and interferometric phased arrays from a functional perspective. It is shown that…