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Related papers: Fluorescence Microscopy: a statistics-optics persp…

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FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

Detection of the air-fluorescence radiation induced by the charged particles of extensive air showers is a well-established technique for the study of ultra-high energy cosmic rays. Fluorescence telescopes provide a nearly calorimetric…

Astrophysics · Physics 2008-07-31 F. Arqueros , F. Blanco , D. Garcia-Pinto , M. Ortiz , J. Rosado

Granular materials are complex multi-particle ensembles in which macroscopic properties are largely determined by inter-particle interactions between their numerous constituents. In order to understand and to predict their macroscopic…

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of…

Quantum optics potentially offers an information channel from the Universe beyond the established ones of imaging and spectroscopy. All existing cameras and all spectrometers measure aspects of the first-order spatial and/or temporal…

Astrophysics · Physics 2015-06-24 Dainis Dravins

Fourier Ptychographic Microscopy (FPM) is a recent technique to overcome the diffraction limit of a low numerical aperture (NA) objective lens by algorithmic post-processing of several lower resolved images. It can increase the…

Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

The evaluation of a photon-pair source employs characteristic metrics like the photon-pair generation rate, heralding efficiency, and second-order correlation function, all of which are determined by the photon number distribution of the…

Quantum Physics · Physics 2024-07-17 Sang Min Lee

We introduce a procedure to automatically count and locate the fluorescent particles in a microscopy image. Our procedure employs an approximate likelihood estimator derived from a Poisson random field model for photon emission. Estimates…

Applications · Statistics 2010-11-10 John Hughes , John Fricks , William Hancock

The identification of light sources represents a task of utmost importance for the development of multiple photonic technologies. Over the last decades, the identification of light sources as diverse as sunlight, laser radiation and…

Quality control in molecular optical sectioning microscopy is indispensable for transforming acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed,…

Integrated spectrophotometric properties of stellar systems are intrinsically dispersed due to the stochastic nature of the small numbers of bright stars they contain. Among clusters, only the most massive ones can be used individually for…

Astrophysics · Physics 2007-05-23 Ariane Lançon , Mustapha Mouhcine

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Lens flare is a common image artifact that can significantly degrade image quality and affect the performance of computer vision systems due to a strong light source pointing at the camera. This survey provides a comprehensive overview of…

Image and Video Processing · Electrical Eng. & Systems 2023-10-24 Yousef Kotp , Marwan Torki

A trade-off between speed and information controls our understanding of astronomical objects. Fast-to-acquire photometric observations provide global properties, while costly and time-consuming spectroscopic measurements enable a better…

Instrumentation and Methods for Astrophysics · Physics 2022-11-11 Lars Doorenbos , Stefano Cavuoti , Giuseppe Longo , Massimo Brescia , Raphael Sznitman , Pablo Márquez-Neila

The rise of interest in the study of quantum emitters has recently prompted many research groups to construct their own confocal epifluorescence microscopy/spectroscopy instruments. The low light levels typically involved in quantum emitter…

Optics · Physics 2022-06-23 K. G. Scheuer , G. J. Hornig , T. R. Harrison , R. G. DeCorby

Intensity, wavevector, phase, and polarization are the most important parameters of any light beam. Understanding the wavevector distribution has emerged as a very important problem in recent days, especially at nanoscale. It provides…

Optics · Physics 2020-01-22 Adarsh B. Vasista , Deepak K. Sharma , G V Pavan Kumar

Spectral and spatial fringes in polarized light are produced by the interference of transmitted and reflected waves at the interface between materials with different indexes of refraction. These instrumental artifacts can affect the…

Optics · Physics 2026-03-11 Roberto Casini , David M. Harrington

The speckle imaging is a photographic technique that resolves objects viewed through severely distorted media. The results are insensitive to the errors caused by apparent size of the isoplanatic patch and the telescope aberrations. In this…

Astrophysics · Physics 2007-05-23 S K Saha