Microscope calibration using laser written fluorescence
Optics
2018-08-29 v1
Abstract
There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.
Cite
@article{arxiv.1806.05457,
title = {Microscope calibration using laser written fluorescence},
author = {Alexander D. Corbett and Michael Shaw and Andrew Yacoot and Andrew Jefferson and Lothar Schermelleh and Tony Wilson and Martin Booth and Patrick S. Salter},
journal= {arXiv preprint arXiv:1806.05457},
year = {2018}
}
Comments
16 pages, 10 figures