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We consider the numerical optimization of performance for a computational extension of a confocal microscope. Using a system where the pinhole detector is replaced with a detector array, we seek to exploit this additional information for…

Signal Processing · Electrical Eng. & Systems 2020-12-15 Keith Dillon

This article experimentally examines different configurations of a novel multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon a densely packed array of "micro-cameras" to jointly image across a large field-of-view…

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…

Optical interferometry is amongst the most sensitive techniques for precision measurement. By increasing the light intensity a more precise measurement can usually be made. However, in some applications the sample is light sensitive. By…

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

Fluorescence microscopy is a widely used method among cell biologists for studying the localization and co-localization of fluorescent protein. For microbial cell biologists, these studies often include tedious and time-consuming manual…

Computer Vision and Pattern Recognition · Computer Science 2020-09-29 Jonathan Reiner , Guy Azran , Gal Hyams

Transition edge sensor microcalorimeters can measure x-ray and gamma-ray energies with very high energy resolution and high photon-collection efficiency. For this technology to reach its full potential in future x-ray observatories, each…

Instrumentation and Methods for Astrophysics · Physics 2017-02-03 J. W. Fowler , B. K. Alpert , W. B. Doriese , D. A. Fischer , C. Jaye , Y. I. Joe , G. C. O'Neil , D. S. Swetz , J. N. Ullom

Common methods to achieve photon number resolution rely on fast on-off single-photon detectors in conjunction with temporal or spatial mode multiplexing. Yet, these methods suffer from an inherent trade-off between the efficiency of photon…

Quantum Physics · Physics 2024-11-21 Anton N. Vetlugin , Filippo Martinelli , Shuyu Dong , Cesare Soci

The development of spectroscopic techniques able to detect and verify quantum coherence is a goal of increasing importance given the rapid progress of new quantum technologies, the advances in the field of quantum thermodynamics, and the…

Quantum Physics · Physics 2020-05-27 Carlos Sánchez Muñoz , Frank Schlawin

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

Several Scientific and engineering applications require merging of sampled images for complex perception development. In most cases, for such requirements, images are merged at intensity level. Even though it gives fairly good perception of…

Computer Vision and Pattern Recognition · Computer Science 2014-08-01 T. R. Gopalakrishnan Nair , Richa Sharma

Lensless imaging is an important and challenging problem. One notable solution to lensless imaging is a single pixel camera which benefits from ideas central to compressive sampling. However, traditional single pixel cameras require many…

Computer Vision and Pattern Recognition · Computer Science 2017-03-31 Guy Satat , Matthew Tancik , Ramesh Raskar

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Size and shape are critical discriminators between molecular species and states. We describe a micro-chip based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution…

Photonic chips that integrate guides, switches, gratings and other components, process vast amounts of information rapidly on a single device. A new branch of this technology becomes possible if the light is coupled to cold atoms in a…

Quantum Physics · Physics 2010-11-23 M. Kohnen , M. Succo , P. G. Petrov , R. A. Nyman , M. Trupke , E. A. Hinds

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

Current methods for detector gain calibration require acquisition of tens of special calibration images. Here we propose a method that obtains the gain from the actual image for which the photon count is desired by quantifying out-of-band…

Instrumentation and Detectors · Physics 2018-03-15 Rainer Heintzmann , Peter K. Relich , Robert P. J. Nieuwenhuizen , Keith A. Lidke , Bernd Rieger

The performance of a confocal imaging system may be no better than a general-purpose widefield system if it is not properly maintained or quality controlled. The publication of ISO 21073, 'Confocal microscopes- Optical data of fluorescence…

Other Quantitative Biology · Quantitative Biology 2020-11-20 Glyn Nelson , Laurent Gelman , Orestis Faklaris , Roland Nitschke , Alex Laude
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