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Purpose: In conventional fluoroscopy-guided interventions, the 2D projective nature of X-ray imaging limits depth perception and leads to prolonged radiation exposure. Virtual fluoroscopy, combined with spatially tracked surgical…
This paper describes the setup of two different solutions for laboratory X-ray microscopy working with geometric magnification. One setup uses thin-film transmission targets with an optimized tungsten-layer thickness and the electron gun…
We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system, and derive a…
Optical coherence tomography (OCT) is a high-resolution three-dimensional imaging technique that enables non-destructive measurements of surface and subsurface microstructures. Recent developments of OCT operating in the mid-infrared (MIR)…
We demonstrate that in situ coherent diffractive imaging (CDI), which harnesses the coherent interference between a strong and a weak beam illuminating a static and dynamic structure, can be a very dose-efficient imaging method. At low…
Many essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents…
Multiexponential modeling of relaxation or diffusion MR signal decays is a popular approach for estimating and spatially mapping different microstructural tissue compartments. While this approach can be quite powerful, it is also limited by…
X-ray nanotomography is a powerful tool for the characterization of nanoscale materials and structures, but is difficult to implement due to competing requirements on X-ray flux and spot size. Due to this constraint, state-of-the-art…
This paper presents SAMSON, a Spectral Absorption-fluorescence Microscopy System for ON-site-imaging of algae within a water sample. Designed to be portable and low-cost for on-site use, the optical sub-system of SAMSON consists of a…
Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…
The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…
Integration of hyperspectral imaging into fluorescence-guided neurosurgery has the potential to improve surgical decision making by providing quantitative fluorescence measurements in real-time. Quantitative fluorescence requires paired…
High-throughput biological imaging is often constrained by a trade-off between acquisition speed and image quality. Fast imaging modalities, such as wide-field fluorescence microscopy, enable large-scale data acquisition but suffer from…
Off-axis X-ray holography is a lensless imaging technique that allows unambiguous retrieval of an object's exit-wave function with high fidelity. It has been used with great success to study nanoscale phenomena and spatio-temporal dynamics…
Although cardiovascular diseases are the leading cause of death globally, non-invasive and inexpensive diagnostic tools for the identification of associated unstable atherosclerotic plaques are not yet available. Scanning acoustic…
X-ray scattering has been an indispensable tool in advancing our understanding of matter, from the first evidence of the crystal lattice to recent discoveries of nuclei's fastest dynamics. In addition to the lattice, ultrafast resonant…
The advent of diffraction limited sources and developments in detector technology opens up new possibilities for the study of materials in situ and operando. Coherent X-ray diffraction techniques such as coherent X-ray diffractive imaging…
The spiral phase contrast microscope can clearly distinguish the morphological information of the low contrast objects (i.e., biological samples) because of the isotropic edge-enhancement effect, while the bright field microscope can image…
Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…