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Single-beam scanning electron microscopes (SEM) are widely used to acquire massive data sets for biomedical study, material analysis, and fabrication inspection. Datasets are typically acquired with uniform acquisition: applying the…

Image and Video Processing · Electrical Eng. & Systems 2021-01-11 Lu Mi , Hao Wang , Yaron Meirovitch , Richard Schalek , Srinivas C. Turaga , Jeff W. Lichtman , Aravinthan D. T. Samuel , Nir Shavit

Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here, we developed a refinement protocol based on Gaussian mixture models that integrates particle…

Quantitative Methods · Quantitative Biology 2023-08-31 Muyuan Chen , Michael F. Schmid , Wah Chiu

A common task in single particle electron cryomicroscopy (cryo-EM) is the rigid alignment of images and/or volumes. In the context of images, a rigid alignment involves estimating the inner-product between one image of $N\times N$ pixels…

Numerical Analysis · Mathematics 2022-12-07 Aaditya V. Rangan

During the acquisition of an image from its source, noise always becomes an integral part of it. Various algorithms have been used in past to denoise the images. Image denoising still has scope for improvement. Visual information…

Image and Video Processing · Electrical Eng. & Systems 2019-09-17 Santosh Paudel , Ajay Kumar Shrestha , Pradip Singh Maharjan , Rameshwar Rijal

A main task in cryo-electron microscopy single particle reconstruction is to find a three-dimensional model of a molecule given a set of its randomly oriented and positioned noisy projection-images. In this work, we propose an algorithm for…

Computational Physics · Physics 2022-11-11 Adi Shasha Geva , Yoel Shkolnisky

Spatially-varying intensity noise is a common source of distortion in medical images. Bias field noise is one example of such a distortion that is often present in the magnetic resonance (MR) images or other modalities such as retina…

Computer Vision and Pattern Recognition · Computer Science 2020-06-18 Reza Abbasi-Asl , Aboozar Ghaffari , Emad Fatemizadeh

The scanning electron microscope (SEM) produces an image of a sample by scanning it with a focused beam of electrons. The electrons interact with the atoms in the sample, which emit secondary electrons that contain information about the…

Computer Vision and Pattern Recognition · Computer Science 2017-09-08 Shahar Tsiper , Or Dicker , Idan Kaizerman , Zeev Zohar , Mordechai Segev , Yonina C. Eldar

A simple, yet general, formalism for the optimized linear combination of astrophysical images is constructed and demonstrated. The formalism allows the user to combine multiple undersampled images to provide oversampled output at high…

Instrumentation and Methods for Astrophysics · Physics 2015-05-28 Barnaby Rowe , Christopher Hirata , Jason Rhodes

Electron microscopy has enabled many scientific breakthroughs across multiple fields. A key challenge is the tuning of microscope parameters based on images to overcome optical aberrations that deteriorate image quality. This calibration…

Computer Vision and Pattern Recognition · Computer Science 2026-03-20 Jilles S. van Hulst , W. P. M. H. Heemels , Duarte J. Antunes

Cryogenic electron microscopy (cryo-EM) has transformed structural biology by allowing to reconstruct 3D biomolecular structures up to near-atomic resolution. However, the 3D reconstruction process remains challenging, as the 3D structures…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Bongjin Koo , Julien Martel , Ariana Peck , Axel Levy , Frédéric Poitevin , Nina Miolane

Depth information plays a crucial role in autonomous systems for environmental perception and robot state estimation. With the rapid development of deep neural network technology, depth estimation has been extensively studied and shown…

Robotics · Computer Science 2024-11-11 Quang Truong Nguyen , Thanh Nguyen Canh , Xiem HoangVan

The bilateral filter has diverse applications in image processing, computer vision, and computational photography. In particular, this non-linear filter is quite effective in denoising images corrupted with additive Gaussian noise. The…

Computer Vision and Pattern Recognition · Computer Science 2015-05-26 Kollipara Rithwik , Kunal Narayan Chaudhury

Low light very likely leads to the degradation of an image's quality and even causes visual task failures. Existing image enhancement technologies are prone to overenhancement, color distortion or time consumption, and their adaptability is…

Image and Video Processing · Electrical Eng. & Systems 2022-05-17 Xiaozhou Lei , Zixiang Fei , Wenju Zhou , Huiyu Zhou , Minrui Fei

State-of-the-art physics experiments require high-resolution, low-noise, and low-threshold detectors to achieve competitive scientific results. However, experimental environments invariably introduce sources of noise, such as electrical…

Cryo-electron microscopy nowadays often requires the analysis of hundreds of thousands of 2D images as large as a few hundred pixels in each direction. Here we introduce an algorithm that efficiently and accurately performs principal…

Computer Vision and Pattern Recognition · Computer Science 2015-12-16 Zhizhen Zhao , Yoel Shkolnisky , Amit Singer

As a hybrid imaging technology, photoacoustic microscopy (PAM) imaging suffers from noise due to the maximum permissible exposure of laser intensity, attenuation of ultrasound in the tissue, and the inherent noise of the transducer.…

Image and Video Processing · Electrical Eng. & Systems 2022-01-13 Da He , Jiasheng Zhou , Xiaoyu Shang , Jiajia Luo , Sung-Liang Chen

The number of noisy images required for molecular reconstruction in single-particle cryo-electron microscopy (cryo-EM) is governed by the autocorrelations of the observed, randomly-oriented, noisy projection images. In this work, we…

Numerical Analysis · Mathematics 2023-05-03 Tamir Bendory , Yuehaw Khoo , Joe Kileel , Oscar Mickelin , Amit Singer

Medical image denoising is considered among the most challenging vision tasks. Despite the real-world implications, existing denoising methods have notable drawbacks as they often generate visual artifacts when applied to heterogeneous…

Image and Video Processing · Electrical Eng. & Systems 2025-03-11 S M A Sharif , Rizwan Ali Naqvi , Woong-Kee Loh

In cryo-electron microscopy (cryo-EM), a microscope generates a top view of a sample of randomly-oriented copies of a molecule. The problem of single particle reconstruction (SPR) from cryo-EM is to use the resulting set of noisy 2D…

Numerical Analysis · Mathematics 2014-09-16 Gene Katsevich , Alexander Katsevich , Amit Singer

Hematoxylin and Eosin (H&E)-stained images are commonly used to detect nuclear or cancerous regions in cells from images captured by a microscope. Identifying cancer cytoplasm is crucial for determining the type of cancer; hence, obtaining…

Image and Video Processing · Electrical Eng. & Systems 2025-07-08 Rebeka Sultana , Hibiki Horibe , Tomoaki Murakami , Ikuko Shimizu