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Weak-signal detection and single-particle selection from low-contrast micrographs of frozen hydrated biomolecules by cryo-electron microscopy (cryo-EM) presents a practical challenge. Cryo-EM image contrast degrades as the size of…

Quantitative Methods · Quantitative Biology 2019-04-16 Youdong Mao , Luis R. Castillo-Menendez , Joseph Sodroski

Error-control-coding (ECC) techniques are widely used in modern digital communication systems to minimize the effect of noisy channels on the quality of received signals. Motivated by the fact that both communication and imaging can be…

Image and Video Processing · Electrical Eng. & Systems 2018-09-20 Xiaopeng Wang , Zunwang Bo , Zihuai Lin , Wenlin Gong , Branka Vucetic , Shensheng Han

We propose a novel method of efficient upsampling of a single natural image. Current methods for image upsampling tend to produce high-resolution images with either blurry salient edges, or loss of fine textural detail, or spurious noise…

Computer Vision and Pattern Recognition · Computer Science 2015-03-03 Chinmay Hegde , Oncel Tuzel , Fatih Porikli

Cryo-electron microscopy (cryo-EM), the subject of the 2017 Nobel Prize in Chemistry, is a technology for determining the 3-D structure of macromolecules from many noisy 2-D projections of instances of these macromolecules, whose…

Computer Vision and Pattern Recognition · Computer Science 2020-04-22 Roy R. Lederman , Joakim Andén , Amit Singer

Discovering the 3D atomic structure of molecules such as proteins and viruses is a fundamental research problem in biology and medicine. Electron Cryomicroscopy (Cryo-EM) is a promising vision-based technique for structure estimation which…

Computer Vision and Pattern Recognition · Computer Science 2016-11-18 Marcus A. Brubaker , Ali Punjani , David J. Fleet

In this paper we study the formal algebraic structure underlying the intrinsic classification algorithm, recently introduced by Hadani, Shkolnisky, Singer and Zhao, for classifying noisy projection images of similar viewing directions in…

Representation Theory · Mathematics 2011-04-07 Ronny Hadani , Amit Singer

Low-light images suffer from severe noise and low illumination. Current deep learning models that are trained with real-world images have excellent noise reduction, but a ratio parameter must be chosen manually to complete the enhancement…

Image and Video Processing · Electrical Eng. & Systems 2020-04-23 Qingxu Fu , Xiaoguang Di , Yu Zhang

The growing role of data-driven approaches to scientific discovery has unveiled a large class of models that involve latent transformations with a rigid algebraic constraint. Three-dimensional molecule reconstruction in Cryo-Electron…

Information Theory · Computer Science 2019-06-04 Amelia Perry , Jonathan Weed , Afonso S. Bandeira , Philippe Rigollet , Amit Singer

Particle picking is a crucial first step in the computational pipeline of single-particle cryo-electron microscopy (cryo-EM). Selecting particles from the micrographs is difficult especially for small particles with low contrast. As…

Computer Vision and Pattern Recognition · Computer Science 2018-08-06 Ayelet Heimowitz , Joakim Andén , Amit Singer

Cryo-electron microscopy (Cryo-EM) enables high-resolution imaging of biomolecules, but structural heterogeneity remains a major challenge in 3D reconstruction. Traditional methods assume a discrete set of conformations, limiting their…

Machine Learning · Statistics 2025-09-09 Diego Sanchez Espinosa , Erik H Thiede , Yunan Yang

Cryo-electron microscopy (cryo-EM) has become a major experimental technique to determine the structures of large protein complexes and molecular assemblies, as evidenced by the 2017 Nobel Prize. Although cryo-EM has been drastically…

Biomolecules · Quantitative Biology 2021-06-01 Dong Si , Andrew Nakamura , Runbang Tang , Haowen Guan , Jie Hou , Ammaar Firozi , Renzhi Cao , Kyle Hippe , Minglei Zhao

The construction of large-scale, high-quality datasets is a fundamental prerequisite for developing robust and generalizable foundation models in motor imagery (MI)-based brain-computer interfaces (BCIs). However, EEG signals collected from…

Computational Engineering, Finance, and Science · Computer Science 2025-06-16 Dingkun Liu , Zhu Chen , Dongrui Wu

A multi-imaging strategy is proposed and experimentally tested to improve the accuracy of photon counting with an electron multiplying charge-coupled device (EMCCD), by taking into account the random nature of its on-chip gain and the…

Astrophysics · Physics 2009-11-13 Eric Lantz , Jean Luc Blanchet , Luca Furfaro , Fabrice Devaux

Cryogenic electron microscopy (cryo-EM) is an invaluable technique for determining high-resolution three-dimensional structures of biological macromolecules using transmission particle images. The inherent symmetry in these macromolecules…

Optimization and Control · Mathematics 2024-11-06 Qi Zhang , Chenglong Bao , Hai Lin , Mingxu Hu

Multifarious image enhancement algorithms have been used in different applications. Still, some algorithms or modules are imperfect for practical use. When the image enhancement modules have been fixed or combined by a series of algorithms,…

Computer Vision and Pattern Recognition · Computer Science 2011-03-03 Lijun Wang , Ling Shao

Cryo-electron microscopy (cryo-EM) is an indispensable technique for determining the 3D structures of dynamic biomolecular complexes. While typically applied to image a single molecular species, cryo-EM has the potential for structure…

Computer Vision and Pattern Recognition · Computer Science 2026-03-23 Jeffrey Gu , Minkyu Jeon , Ambri Ma , Serena Yeung-Levy , Ellen D. Zhong

Due to numerous hardware shortcomings, medical image acquisition devices are susceptible to producing low-quality (i.e., low contrast, inappropriate brightness, noisy, etc.) images. Regrettably, perceptually degraded images directly impact…

Image and Video Processing · Electrical Eng. & Systems 2025-03-12 S M A Sharif , Rizwan Ali Naqvi , Mithun Biswas , Woong-Kee Loh

Single-particle cryo-EM has transformed structural biology but still faces challenges in resolving conformational heterogeneity at atomic resolution. Existing cryo-EM heterogeneity analysis methods either lack atomic details or tend to…

Biomolecules · Quantitative Biology 2025-10-21 Chengwei Zhang , Shimian Li , Yihao Niu , Zhen Zhu , Sihao Yuan , Sirui Liu , Yi Qin Gao

Capturing images under extremely low-light conditions poses significant challenges for the standard camera pipeline. Images become too dark and too noisy, which makes traditional enhancement techniques almost impossible to apply. Recently,…

Computer Vision and Pattern Recognition · Computer Science 2021-11-23 Ahmet Serdar Karadeniz , Erkut Erdem , Aykut Erdem

When one captures images in low-light conditions, the images often suffer from low visibility. This poor quality may significantly degrade the performance of many computer vision and multimedia algorithms that are primarily designed for…

Computer Vision and Pattern Recognition · Computer Science 2016-07-26 Xiaojie Guo
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