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Automated segmentation of retinal blood vessels in label-free fundus images entails a pivotal role in computed aided diagnosis of ophthalmic pathologies, viz., diabetic retinopathy, hypertensive disorders and cardiovascular diseases. The…

Computer Vision and Pattern Recognition · Computer Science 2016-11-17 Avisek Lahiri , Abhijit Guha Roy , Debdoot Sheet , Prabir Kumar Biswas

Accurate assessment of tissue perfusion is crucial in visceral surgery, especially during anastomosis. Currently, subjective visual judgment is commonly employed in clinical settings. Hyperspectral imaging (HSI) offers a non-invasive,…

Image and Video Processing · Electrical Eng. & Systems 2025-04-16 Stefan Kray , Andreas Schmid , Eric L. Wisotzky , Moritz Gerlich , Sebastian Apweiler , Anna Hilsmann , Thomas Greiner , Peter Eisert , Werner Kneist

Significance: A path is described to increase the sensitivity and accuracy of body-worn devices used to monitor patient health. This path supports improved health management. A wavelength-choice algorithm developed at Mayo demonstrates that…

Applied Physics · Physics 2023-01-10 Barry K. Gilbert , Clifton R. Haider , Daniel J. Schwab , Gary S. Delp

In computational pathology, multiple instance learning (MIL) is widely used to circumvent the computational impasse in giga-pixel whole slide image (WSI) analysis. It usually consists of two stages: patch-level feature extraction and…

Image and Video Processing · Electrical Eng. & Systems 2023-09-21 Beidi Zhao , Wenlong Deng , Zi Han , Li , Chen Zhou , Zuhua Gao , Gang Wang , Xiaoxiao Li

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

We present a rapid, large-field bimodal imaging platform that integrates conventional brightfield microscopy with a lensless IR imaging scanner, enabling whole-slide IR image stack acquisition in minutes. Using a dedicated deep learning…

Sonography techniques use multiple transducer elements for tissue visualization. Signals detected at each element are sampled prior to digital beamforming. The required sampling rates are up to 4 times the Nyquist rate of the signal and…

Information Theory · Computer Science 2012-12-21 T. Chernyakova , Y. C. Eldar , R. Amit

In immunology studies, flow cytometry is a commonly used multivariate single-cell assay. One key goal in flow cytometry analysis is to pinpoint the immune cells responsive to certain stimuli. Statistically, this problem can be translated…

Methodology · Statistics 2021-03-29 John Pura , Xuechan Li , Cliburn Chan , Jichun Xie

Lensless imaging is an elegant approach to high-resolution microscopy, which is rapidly gaining popularity in applications where imaging optics are problematic. However, current lensless imaging methods require objects to be placed within a…

Phase-contrast tomographic flow cytometry combines quantitative 3D analysis of unstained single cells and high-throughput. A crucial issue of this method is the storage and management of the huge amount of 3D tomographic data. Here we show…

Conventionally, 3-dimensional cellular tomography can be done with light sheet or multi-angle observations. Recently, a new technique was introduced where the cell was rotated using convection currents to visualize the outer periphery (Liu…

A flow-through sensing platform based on open-ended porous silicon (PSi) microcavity membranes that are compatible with integration in on-chip sensor arrays is demonstrated. Due to the high aspect ratio of PSi nanopores, the performance of…

Materials Science · Physics 2016-10-27 Yiliang Zhao , Girija Gaur , Scott T. Retterer , Paul E. Laibinis , Sharon M. Weiss

Optical endoscopy plays a crucial role in minimally invasive medical diagnostics and therapeutic procedures. Nonetheless, state-of-the-art endoscopic zoom objectives are bulky, requiring multi-element lens actuation, which can limit…

Multiphase flows where particles, bubbles, or droplets are suspended in a fluid govern critical processes in biology, medicine, materials processing, and geophysics. However, observing their microscale dynamics in opaque systems has…

We introduce a machine-learning-based approach to enhance the sensitivity of optical-extreme ultraviolet (XUV) transient absorption spectroscopy. A reference spectrum is used as input to a three-layer feed-forward neural network, allowing…

Optics · Physics 2023-05-04 Tobias Heinrich , Hung-Tzu Chang , Sergey Zayko , Murat Sivis , Claus Ropers

Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines Fourier ptychography with waveguide microscopy to realize a…

Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there have been no techniques reported for snapshot imaging of thick samples in large…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Non-invasive measurement of the arterial blood speed gives rise to important healthinformation such as cardio output and blood supplies to vital organs. The magnitude andchange in arterial blood speed are key indicators of the health…

Medical Physics · Physics 2021-10-22 Alex Ce Zhang , Yu-Hwa Lo

High-efficiency optical detectors that can determine the number of photons in a pulse of monochromatic light have applications in a variety of physics studies, including post-selection-based entanglement protocols for linear optics quantum…

Quantum Physics · Physics 2009-11-11 Danna Rosenberg , Adriana E. Lita , Aaron J. Miller , Sae Woo Nam