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Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis…

Complex field imaging, which captures both the amplitude and phase information of input optical fields or objects, can offer rich structural insights into samples, such as their absorption and refractive index distributions. However,…

Optics · Physics 2024-05-30 Jingxi Li , Yuhang Li , Tianyi Gan , Che-Yung Shen , Mona Jarrahi , Aydogan Ozcan

Lipid droplets (LDs), once considered mere storage depots for lipids, have gained recognition for their intricate roles in cellular processes, including metabolism, membrane trafficking, and disease states like obesity and cancer. This…

Biological Physics · Physics 2023-10-26 Hyeonwoo Kim , Seungeun Oh , Seongsoo Lee , Kwang suk Lee , YongKeun Park

Contrast-enhanced imaging is central to oncologic diagnosis, but contrast agents can be contraindicated for many of the patients who need them most. Synthesizing contrast scans from non-contrast inputs is the natural response. Two obstacles…

Computer Vision and Pattern Recognition · Computer Science 2026-05-11 Yifan Chen , Fei Yin , Hao Chen , Jia Wu , Chao Li

We report the experimental demonstration of deeply subwavelength far-field optical imaging of unlabelled samples with resolution better than $\lambda/20$. We beat the ~$\lambda$/2 diffraction limit of conventional optical microscopy several…

Optics · Physics 2020-01-07 Tanchao Pu , Jun-Yu Ou , Nikitas Papasimakis , Nikolay I. Zheludev

We demonstrate pixelation-free real-time widefield endoscopic imaging through an aperiodic multicore fiber (MCF) without any distal opto-mechanical elements or proximal scanners. Exploiting the memory effect in MCFs the images in our system…

Interferometric imaging is a well established method to image phase objects by mixing the image wavefront with a reference one on a CCD camera. It has also been applied to fast transient phenomena, mostly through the analysis of single…

A lateral shear interferometer based digital holographic microscopy has been realized to study the morphology dynamics of Human red blood cells quantitatively. Here, a lateral shear interferometer is embedded with a conventional microscope…

Image and Video Processing · Electrical Eng. & Systems 2019-09-10 Shital Devinder , Ashish Lal , Tathagato Rai Dastidar , Satish Kumar Dubey

Pump-probe microscopy enables label-free imaging of structural and chemical features of samples. However, signals in pump-probe microscopy are typically small and often must be measured in the presence of large backgrounds. As a result,…

A manual measurement of blood vessels diameter is a conventional component of routine visual assessment of microcirculation, say, during optical capillaroscopy. However, many modern optical methods for blood flow measurements demand the…

Quantitative Methods · Quantitative Biology 2017-04-21 Eugene B. Postnikov , Maria O. Tsoy , Maxim A. Kurochkin , Dmitry E. Postnov

Optical coherence tomography angiography (OCTA) has been established as a powerful tool for investigating vascular diseases and is expected to become a standard of care technology. However, its widespread clinical usage is hindered by…

Medical Physics · Physics 2021-09-10 Si Chen , Kan Lin , Linbo Liu

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

Quantitative Methods · Quantitative Biology 2025-11-03 Surajit Das , Pavel Zun

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

We have designed optical metasurfaces hosting high-quality factor quasi-bound state in the continuum (q-BIC) resonances for optical biosensing in flow. The unit cell of the metasurface contains two rectangular bars. An asymmetry factor is…

This work proposes lossless and near-lossless compression algorithms for multi-channel biomedical signals. The algorithms are sequential and efficient, which makes them suitable for low-latency and low-power signal transmission…

Information Theory · Computer Science 2016-05-17 Ignacio Capurro , Federico Lecumberry , Álvaro Martín , Ignacio Ramírez , Eugenio Rovira , Gadiel Seroussi

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…

Optics · Physics 2023-08-04 Herve Hugonnet , HyunJun Han , Weisun Park , YongKeun Park

We present the development and validation of a novel multimodal optical imaging platform that integrates hyperspectral imaging (HSI) and laser speckle contrast imaging (LSCI) to enable real-time, non-invasive mapping of tissue oxygenation,…

Quantum sensing with undetected photons is a technique where photons of one wavelength probe a sample, but information is extracted by measuring photons of another wavelength that never interacts with the sample. This has seen significant…

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