Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index
Quantitative Methods
2017-04-17 v1 Biological Physics
Optics
Abstract
Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis of three-dimensional (3D) Fourier components of light diffracted by the sample. This leads to the direct recovery of quantitative cellular morphology with no iterative procedures for reduced computational complexity. Combing with the fact that the technique is easily adaptive to any imaging platform and requires minimum sample preparation, our proposed method is particularly promising for observing fast, volumetric and dynamic events previously only accessible through staining methods.
Keywords
Cite
@article{arxiv.1704.04445,
title = {Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index},
author = {Chen Liu and Michael Malek and Ivan K. H. Poon and Lanzhou Jiang and Colin J. R. Sheppard and Ann Roberts and Harry Quiney and Douguo Zhang and Xiaocong Yuan and Jiao Lin and Christian Depeursinge and Pierre Marquet and Shan Shan Kou},
journal= {arXiv preprint arXiv:1704.04445},
year = {2017}
}
Comments
20 pages, 10 figures