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We present a new physical model resolving a long-standing mystery of the power-law distributions of the blinking times in single colloidal quantum dot fluorescence. The model considers the non-radiative relaxation of the exciton through…
Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…
Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence…
We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or…
Diffraction is a manifestation of light at edge due to its wavelike nature. The well-known diffraction phenomena are Fresnel and Fraunhofer, they find variety of applications individually. But the synergy of two phenomena is not studied and…
We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarization of the illumination or the detection…
We present the starblade algorithm, a method to separate superimposed point sources from auto-correlated, diffuse flux using a Bayesian model. Point sources are assumed to be independent from each other and to follow a power-law brightness…
We show that the output mode of a single-mode optical fiber can be directly focused to a sub-wavelength waist with a finite working distance by tapering the fiber to a diameter of the order of the wavelength and terminating it with a…
Current optical manipulation techniques rely on carefully engineered setups and samples. Although similar conditions are routinely met in research laboratories, it is still a challenge to manipulate microparticles when the environment is…
The spontaneous fluorescence rates of single-molecule emitters are typically on the order of nanoseconds. However coupling them with plasmonic nanostructures can substantially increase their fluorescence yields. The confinement between the…
The diffusion of macromolecules in cells and in complex fluids is often found to deviate from simple Fickian diffusion. One explanation offered for this behavior is that molecular crowding renders diffusion anomalous, where the mean-squared…
We reveal an unreported physical mechanism that enables the pre-concentration, sorting and characterization of charged polystyrene nanobeads and liposomes dispersed in a continuous flow within a straight micron-sized channel. Initially, a…
Single-file diffusion is a ubiquitous physical process exploited by living and synthetic systems to exchange molecules with their environment. It is paramount quantifying the escape time needed for single files of particles to exit from…
Point spread function (PSF) plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view as astigmatism, coma and vignetting. Thus, one should…
Analysis of thermal dust emission spectra for dust mineralogy and physical grain properties depends on laboratory-measured or calculated comparison spectra. Often, the agreement between these two kinds of spectra is not satisfactory because…
The selective optical detection of individual metallic nanoparticles (NPs) with high spatial and temporal resolution is a challenging endeavour, yet is key to the understanding of their optical response and their exploitation in…
We present PyGFit, a program designed to measure PSF-matched photometry from images with disparate pixel scales and PSF sizes. While PyGFit has a number of uses, its primary purpose is to extract robust spectral energy distributions (SEDs)…
Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…
In single molecule orientation localization microscopy, valuable information about the orientation and longitudinal position of each molecule is often encoded in the shape of the point spread function (PSF). This shape, though, can be…
We show that the errors due to atmospheric refraction are present in the magnitudes determined with the Difference Images Analysis method. In case of single, unblended stars the size of the effect agrees with the theoretical prediction. But…