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Pioneering methods in recent optical lattice experiments allow to focus laser beams down to a spot size that is comparable to the lattice constant. Inspired by this achievement, we examine the resonance fluorescence spectra of two-level…
Spectroastrometry is a technique which has the potential to resolve flux distributions on scales of milliarcseconds. In this study, we examine the application of spectroastrometry to binary point sources which are spatially unresolved due…
Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…
Diffraction experiments have moved to ever heavier objects in recent years, now standing at the level of large molecules. Experiments in materials science on the other hand have come down to ever smaller sizes largely due to the success of…
The point spread function (PSF) reflects states of a telescope and plays an important role in development of data processing methods, such as PSF based astrometry, photometry and image restoration. However, for wide field small aperture…
We use molecules to couple light into and out of microscale plasmonic waveguides. Energy transfer, mediated by surface plasmons, from donor molecules to acceptor molecules over ten micrometer distances is demonstrated. Also surface plasmon…
Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool for analyzing diffusion and binding processes. Confocal scanning laser microscopes that are used in FRAP experiments bleach regions with a radially…
Multi-mode fiber (MMF) endoscopes are a new type of endoscope that use only a single optical fiber to transmit images, achieving much higher spatial resolution for a given diameter than commercial fiber bundle endoscopes. However, a…
Interferometric particle imaging is a widely used optical measuring technique for the sizing of poly-dispersed spherical particles like droplets and bubbles. In its conventional approach, the method is limited to forward-scattering angles…
Randomly rotating particles that have been isotropically labeled with rigidly linked fluorophores will undergo non-isotropic (patchy) photobleaching under illumination due to the dipole coupling of fluorophores with light. For a rotational…
An appealing definition of the term "molecule" arises from consideration of the nature of fluorescence, with discrete molecular entities emitting a stream of single photons. We address the question of how large a molecular object may become…
An increasing number of research topics and applications ask for a precise measurement of the size distribution of small bubbles in a liquid - and hence for reliable and automated image analysis. However, due to the strong mismatch between…
Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photo-thermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying…
Recording the fluorescence of a magneto-optical trap (MOT) is a standard tool for measuring atom numbers in experiments with ultracold atoms. When trapping few atoms in a small MOT, the emitted fluorescence increases with the atom number in…
We present the implementation and use of algorithms for matching point-spread functions (PSFs) within the Pan-STARRS Image Processing Pipeline (IPP). PSF-matching is an essential part of the IPP for the detection of supernovae and…
Fluorescence resonance energy transfer (FRET) from individual, dye-labeled RNA molecules confined in freely-diffusing attoliter-volume aqueous droplets is carefully compared to FRET from unconfined RNA in solution. The use of…
Single-molecule stretching experiments on DNA, RNA, and other biological macromolecules opened up the possibility of an impressive progress in many fields of Life and Medical sciences. The reliability of such experiments may be crucially…
Particle plasmons in metal nanoparticles have primarily been investigated through the use of local optical response approximations. However, as nanoparticle size approaches the average distance of electrons to the metal surface, mesoscopic…
Accurate blur estimation is essential for high-performance imaging across various applications. Blur is typically represented by the point spread function (PSF). In this paper, we propose a physics-informed PSF learning framework for…
In this work we derive analytic expressions and numerical recipes for finding the effective observed position of sources close enough on sky that their Point Spread Functions (PSF), modelled as Gaussian profiles, overlap. In particularly we…