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The measurement of circular dichroism (CD) has widely been exploited to distinguish the different enantiomers of chiral structures. It has been applied to natural materials (e.g. molecules) as well as to artificial materials (e.g.…

Structured illumination microscopy (SIM) is a wide-field super-resolution technique normally limited to roughly twice the diffraction-limited resolution ($\approx 100$--$200$~nm). Surpassing this bound is a classic ill-posed inverse…

Sub-diffraction resolution, gentle sample illumination, and the possibility to image in multiple colors make Structured Illumination Microscopy (SIM) an imaging technique which is particularly well suited for live cell observations. Here,…

Controlling optical chirality at the subwavelength scales is essential for many applications of nanophotonic structures in polarization optics, sensing, and nonlinear photonics. Achieving a strong chiroptical response in planar dielectric…

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

Image and Video Processing · Electrical Eng. & Systems 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

Instrumentation and Detectors · Physics 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

Since the invention of digital cameras there has been a concerted drive towards detector arrays with higher spatial resolution. Microscanning is a technique that provides a final higher resolution image by combining multiple images of a…

Multimodal and multi-information microscopy techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) extend the informational channels beyond intensity-based fluorescence microscopy but suffer from reduced image quality due to…

Image and Video Processing · Electrical Eng. & Systems 2025-04-15 Hao Chen , Julian Najera , Dagmawit Geresu , Meenal Datta , Cody Smith , Scott Howard

Scanning acoustic microscopy (SAM) has been employed since microscopic images are widely used for biomedical or materials research. Acoustic imaging is an important and well-established method used in nondestructive testing (NDT),…

Image and Video Processing · Electrical Eng. & Systems 2022-08-09 Shubham Kumar Gupta , Azeem Ahmad , Prakhar Kumar , Frank Melandso , Anowarul Habib

Patient scans from MRI often suffer from noise, which hampers the diagnostic capability of such images. As a method to mitigate such artifact, denoising is largely studied both within the medical imaging community and beyond the community…

Image and Video Processing · Electrical Eng. & Systems 2022-03-25 Hyungjin Chung , Eun Sun Lee , Jong Chul Ye

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Chirality refers to a geometric phenomenon in which objects are not superimposable on their mirror image. Structures made of nano-scale chiral elements can display chiroptical effects, such as dichroism for left- and right- handed…

Optics · Physics 2020-02-25 Behrooz Semnani , Jeremy Flannery , Rubayet Al Maruf , Michal Bajcsy

Increasing the sensitivity of chiral spectroscopic techniques such as circular dichroism (CD) spectroscopy is a current aspiration in the research field of nanophotonics. Enhancing CD spectroscopy depends upon of two complementary…

Scanning Electron Microscopy (SEM) is critical in nanotechnology, materials science, and biological imaging due to its high spatial resolution and depth of focus. Signal-to-noise ratio (SNR) is an essential parameter in SEM because it…

Machine Learning · Computer Science 2025-10-10 K. S. Sim , I. Bukhori , D. C. Y. Ong , K. B. Gan

Conventional linear optical activity effects are widely used for studying chiral materials. However, poor contrast and artifacts due to sample anisotropy limit the applicability of these methods. Here we demonstrate that nonlinear…

Biological Physics · Physics 2016-02-16 Mei-Yu Chen , Mikko J. Huttunen , Che-Wei Kan , Yen-Yin Lin , Cin-Wei Ye , Meng-Jer Wu , Hsiang-Lin Liu , Shi-Wei Chu

In fluorescence microscopy live-cell imaging, there is a critical trade-off between the signal-to-noise ratio and spatial resolution on one side, and the integrity of the biological sample on the other side. To obtain clean high-resolution…

Image and Video Processing · Electrical Eng. & Systems 2020-08-25 Ruofan Zhou , Majed El Helou , Daniel Sage , Thierry Laroche , Arne Seitz , Sabine Süsstrunk

Large optical chirality in the vicinity of achiral high index dielectric nanostructures has been recently demonstrated as useful means of enhancing molecular circular dichroism. We theoretically study the spatial dependence of optical…

Optics · Physics 2022-05-25 Krzysztof M. Czajkowski , Tomasz J. Antosiewicz

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

We propose a deconvolution algorithm for images blurred and degraded by a Poisson noise. The algorithm uses a fast proximal backward-forward splitting iteration. This iteration minimizes an energy which combines a \textit{non-linear} data…

Applications · Statistics 2008-12-18 François-Xavier Dupé , Jalal Fadili , Jean Luc Starck