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In the close vicinity of a chiral nanostructure, the circular dichroism of a biomolecule could be greatly enhanced, due to the interaction with the local superchiral fields. Modest enhancement of optical activity using a planar…

Resonant chiral dielectric metasurfaces can support circular dichroism exceeding that of natural materials, but their small dissipative losses simultaneously limit the maximization of circular dichroism, which inherently relies on…

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…

Image and Video Processing · Electrical Eng. & Systems 2026-03-19 Jiaming Qian , Jing Feng , Hongjun Wu , Maoxian Zhang , Dongqin Lu , Tianchi Kang , Xinyu Han , Qian Chen , Chao Zuo

We present a study that evaluates the impact of regularization on the achieved resolution in restorations from a novel three-dimensional (3D) Structured Illumination Microscopy (3D-SIM) system with desirable tunability properties. This…

Image and Video Processing · Electrical Eng. & Systems 2024-03-22 Arash Atibi , Abdulaziz Alqahtani , Mohammed Younis , Chrysanthe Preza

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in…

Correlation plenoptic imaging (CPI) is a scanning-free diffraction-limited 3D optical imaging technique exploiting the peculiar properties of correlated light sources. CPI has been further extended to samples of interest to microscopy, such…

Circular dichroism (CD) sensing plays a pivotal role in probing molecular chirality in biomedical sciences. However, engineering superchiral electromagnetic fields that can reliably amplify the faint signatures of chiral analytes remains…

The iterative refinement method (IRM) has been very successfully applied in many different fields for examples the modern quantum chemical calculation and CT image reconstruction. It is proved that the refinement method can create an exact…

Medical Physics · Physics 2015-12-23 Kang Yang , Kevin Yang , Xintie Yang , Shuang-Ren Zhao

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…

We perform a theoretical and numerical study of the optical properties of both direct and inverse three-dimensional (3D) chiral woodpile structures, and a corresponding chiral Bragg stack. We compute transmission spectra in the helical…

Microscopy is routinely used to image biological structures of interest. Due to imaging constraints, acquired images, also called as micrographs, are typically low-SNR and contain noise. Over the last few years, regression-based tasks like…

Image and Video Processing · Electrical Eng. & Systems 2024-10-16 Ashesh Ashesh , Joran Deschamps , Florian Jug

Three-dimensional (3D) fluorescence imaging provides a vital approach for study of biological tissues with intricate structures, and optical sectioning structured illumination microscopy (OS-SIM) stands out for its high imaging speed, low…

DNA and proteins are chiral: their three-dimensional structure cannot be superimposed with its mirror image. Circular dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case…

Biomolecules · Quantitative Biology 2020-09-09 Steven Daly , Frederic Rosu , Valerie Gabelica

Structured illumination microscopy (SIM) can double the resolution beyond the light diffraction limit, but it comes at the cost of multiple camera exposures and the heavy computation burden of multiple Fourier transforms. In this paper, we…

Image and Video Processing · Electrical Eng. & Systems 2021-07-14 Qinnan Zhang , En Bo , Jiawei Chen , Jiaosheng Li , Heming Jiang , Xiaoxu Lu , Liyun Zhong , Jindong Tian

Structured illumination microscopy (SIM) can achieve a $2\times$ resolution enhancement beyond the classical diffraction limit by employing illumination translations with respect to the object. This method has also been successfully…

Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles, which plays a vital role in studying the molecular structure and dynamics of bio-complex. However, it is difficult to resolve the dipole…