Resonant second-harmonic-generation circular-dichroism microscopy reveals molecular chirality in native biological tissues
Biological Physics
2016-02-16 v1 Optics
Abstract
Conventional linear optical activity effects are widely used for studying chiral materials. However, poor contrast and artifacts due to sample anisotropy limit the applicability of these methods. Here we demonstrate that nonlinear second-harmonic-generation circular dichroism spectral microscopy can overcome these limits. In intact collagenous tissues, clear spectral resonance is observed with sub-micrometer spatial resolution. By performing gradual protein denaturation studies, we show that the resonant responses are dominantly due to the molecular chirality.
Keywords
Cite
@article{arxiv.1602.04753,
title = {Resonant second-harmonic-generation circular-dichroism microscopy reveals molecular chirality in native biological tissues},
author = {Mei-Yu Chen and Mikko J. Huttunen and Che-Wei Kan and Yen-Yin Lin and Cin-Wei Ye and Meng-Jer Wu and Hsiang-Lin Liu and Shi-Wei Chu},
journal= {arXiv preprint arXiv:1602.04753},
year = {2016}
}