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Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay…

Optics · Physics 2012-08-28 Ke Si , Reto Fiolka , Meng Cui

We present the Phase A Science Case for the Multi-conjugate Adaptive-optics Visible Imager-Spectrograph (MAVIS), planned for the Adaptive Optics Facility (AOF) of the Very Large Telescope (VLT). MAVIS is a general-purpose instrument for…

We propose a novel coaxial spherical-lens shaping system to achieve a flat-top focus profile by exploiting the spherical aberration of the lens. The well-known aspherical-lens shaping system is compared to ours, and a comparison of etching…

Optics · Physics 2023-03-01 Wei Yang , Zhen Zhang

As a general rule of thumb the resolution of a light microscope (i.e. the ability to discern objects) is predominantly described by the full width at half maximum (FWHM) of its point spread function (psf)---the diameter of the blurring…

Statistics Theory · Mathematics 2020-10-23 Gytis Kulaitis , Axel Munk , Frank Werner

Flow cytometry is a widespread and powerful technique, whose resolution is determined by its capacity to accurately distinguish fluorescently positive populations from negative ones. However, most informative results are discarded while…

The Far Ultraviolet Spectroscopic Explorer satellite observes light in the far-ultraviolet spectral region, 905 - 1187 A with high spectral resolution. The instrument consists of four coaligned prime-focus telescopes and Rowland…

Astrophysics · Physics 2009-10-31 H. W. Moos

Perfect lensing using negative refractive index materials and radiationless electromagnetic interference both provide extreme subwavelength focusing by "amplifying" evanescent wave components that are usually lost. This paper provides a…

Optics · Physics 2015-06-03 Reuven Gordon

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

Diffraction limits the behaviour of light in optical systems and sets the smallest achievable line width at half the wavelength. With a novel subwavelength plasmonic lens to reduce the diffraction via an asymmetry and to generate and…

Optics · Physics 2009-01-30 K. R. Chen

X-ray and optical observations of quadruply lensed quasars can provide a microarcsecond probe of the lensed quasar, corresponding to scale sizes of \~10^2-10^4 gravitational radii of the central black hole. This high angular resolution is…

Astrophysics · Physics 2011-02-11 David Pooley , Jeffrey A. Blackburne , Saul Rappaport , Paul L. Schechter

A lens performs an approximately one-to-one mapping from the object to the image planes. This mapping in the image plane is maintained within a depth of field (or referred to as depth of focus, if the object is at infinity). This…

The performance of light-field microscopy is improved by selectively illuminating the relevant subvolume of the specimen with a second objective lens [1-3]. Here we advance this approach to a single-objective geometry, using an oblique…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

Advances in fluorescence microscopy have reached a plateau in improving depth-to-resolution ratios for imaging scattering tissues, highlighting an urgent need for innovative techniques. We introduce Wavelens, a groundbreaking opaque lens…

Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of…

Optics · Physics 2019-02-06 Lyubov V. Amitonova

Hair-thin optical fiber endoscopes have opened up new paradigms for advanced imaging applications in vivo. In certain applications, such as optical coherence tomography (OCT), light-shaping structures may be required on fiber facets to…

Multiview images have flexible field of view (FoV) but inflexible depth of field (DoF). To overcome the limitation of multiview images on visual tasks, in this paper, we present varifocal multiview (VFMV) images with flexible DoF. VFMV…

Image and Video Processing · Electrical Eng. & Systems 2021-11-22 Kejun Wu , Qiong Liu , Guoan Li , Gangyi Jiang , You Yang

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…