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Light-sheet fluorescence microscopy (LSFM) makes use of a thin plane of light to optically section and image transparent tissues or organisms {\it{in vivo}}, which has the advantages of fast imaging speed and low phototoxicity. In this…

Biological Physics · Physics 2022-09-12 Hong Ye , Xin Xu , Jixiang Wang , Jing Wang , Yi He , Yu Mu , Guohua Shi

Investigation of external flows past arbitrary objects requires access to the information in the boundary layer and the inviscid flow to paint a full picture of their characteristics. However, in laser diagnostic techniques such as particle…

Fluid Dynamics · Physics 2023-10-09 Shuangjiu Fu , Shabnam Raayai-Ardakani

Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…

Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…

We describe the apparatus of a fluorescence optical microscope with both single-photon and two-photon non-diffracting light sheets excitation for large volume imaging. With special design to accommodate two different wavelength ranges…

Instrumentation and Detectors · Physics 2018-04-04 Sze Cheung Lau , Hoi Chun Chiu , Luwei Zhao , Teng Zhao , M. M. T. Loy , Shengwang Du

Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot…

Biological Physics · Physics 2015-01-05 Rumelo Amor , Sumeet Mahajan , William Bradshaw Amos , Gail McConnell

In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging performance. Two-photon LSFM (2P-LSFM), in…

We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…

Instrumentation and Detectors · Physics 2011-06-17 Jan A. N. Buytaert , Emilie Descamps , Dominique Adriaens , Joris J. J. Dirckx

Thin structured surfaces allow flexible control over propagation of electromagnetic waves. Focusing and polarization state analysis are among functions, required for effective manipulation of radiation. Here a polarization sensitive Fresnel…

Optics · Physics 2018-05-23 Hen Markovich , Dmitrii Filonov , Ivan Shishkin , Pavel Ginzburg

High efficiency collection of photons emitted by a point source over a wide field-of-view (FoV) is crucial for many applications. Multi-scale optics over improved light collection by utilizing small optical components placed close to the…

Light-sheet fluorescence microscopy (LSFM), a planar illumination technique that enables high-resolution imaging of samples, experiences defocused image quality caused by light scattering when photons propagate through thick tissues. To…

Image and Video Processing · Electrical Eng. & Systems 2023-11-06 Yu Liu , Gesine Muller , Nassir Navab , Carsten Marr , Jan Huisken , Tingying Peng

In this paper, a modified Maxwell's fish-eye lens is proposed in order to achieve super-resolution imaging. This lens possesses elevated refractive index profile compared with the traditional Maxwell's fish-eye lens. The refractive index…

Classical Physics · Physics 2024-02-08 Liuxian Zhao , Chunlin Li , Xuxu Zhuang , Hao Guo , Yongquan Liu

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…

Conventional light focusing, i. e. concentration of an extended optical field within a small area around a point, is a frequently used process in Optics. An important extension to conventional focusing is the generation of the annular focal…

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

Laser writing of materials is normally performed by the sequential scanning of a single focused beam across a sample. This process is time-consuming and it can severely limit the throughput of laser systems in key applications such as…

Applied Physics · Physics 2019-07-22 A. Zunino , S. Surdo , M. Duocastella

Imaging with a layered superlens is a spatial filtering operation characterized by the point spread function (PSF). We show that in the same optical system the image of a narrow sub-wavelength Gaussian incident field may be surprisingly…

Optics · Physics 2010-04-08 Rafal Kotynski , Tomasz Stefaniuk

Wide field-of-view (FOV) optics are widely used in various imaging, display, and sensing applications. While conventional wide FOV optics rely on cascading multiple elements to suppress coma and other aberrations, it has recently been…

Optics · Physics 2021-08-25 Fan Yang , Sensong An , Mikhail Y. Shalaginov , Hualiang Zhang , Juejun Hu , Tian Gu

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

In principle, diffractive optics, particularly Phase Fresnel Lenses (PFLs), offer the ability to construct large, diffraction-limited, and highly efficient X-ray/$\gamma$-ray telescopes, leading to dramatic improvement in angular resolution…