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Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be…

Quantitative Methods · Quantitative Biology 2018-01-03 Paul Müller , Mirjam Schürmann , Chii J. Chan , Jochen Guck

Optical diffraction tomography (ODT) has emerged as an important label-free tool in biomedicine to measure the three-dimensional (3D) structure of a biological sample. In this paper, we describe ODT using second-harmonic generation (SHG)…

Optics · Physics 2024-05-21 Amirhossein Saba , Carlo Gigli , Ye Pu , Demetri Psaltis

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

We propose and experimentally demonstrate temporally low-coherent optical diffraction tomography (ODT) based on angle-scanning Mach-Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, we successfully…

Optics · Physics 2018-07-17 KyeoReh Lee , Seungwoo Shin , Zahid Yaqoob , Peter T. C. So , YongKeun Park

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

Image and Video Processing · Electrical Eng. & Systems 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

Optical diffraction tomography (ODT) is a powerful technique for quantitative, label-free reconstruction of the three-dimensional refractive index (RI) distribution of biological samples. While ODT is well established for imaging thin,…

We present a novel illumination control technique for optical diffraction tomography (ODT). Various spatial frequencies of beam illumination were controlled by displaying time-averaged sinusoidal patterns using a digital micromirror device…

Biological Physics · Physics 2017-04-05 Kyeoreh Lee , Kyoohyun Kim , Geon Kim , Seungwoo Shin , YongKeun Park

Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral…

Optical diffraction tomography (ODT) is a three-dimensional (3D) quantitative phase imaging technique, which enables the reconstruction of the 3D refractive index (RI) distribution of a transparent sample. Due to its fast, non-invasive, and…

Optics · Physics 2018-11-14 Chansuk Park , Seungwoo Shin , Yongkeun Park

Holographic Tomography (HT) or Optical Diffraction Tomography (ODT) provides slice-by-slice information about the refractive index (RI) of three-dimensional (3D) samples and is emerging as an important label-free imaging modality for Life…

Image and Video Processing · Electrical Eng. & Systems 2025-04-22 N Goyal , K Khare

Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials…

Many biological and soft matter processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding experimental challenge. Here, we introduce Fourier…

Optical coherence tomography (OCT) has seen widespread success as an in vivo clinical diagnostic 3D imaging modality, impacting areas including ophthalmology, cardiology, and gastroenterology. Despite its many advantages, such as high…

Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has the limited access to…

Optics · Physics 2017-11-22 Moosung Lee , Seungwoo Shin , YongKeun Park

We demonstrate a three-dimensional (3D) optical diffraction tomographic technique with optimal frequency combination (OFC-ODT) for the 3D quantitative phase imaging of unlabeled specimens. Three sets of through-focus intensity images are…

Optics · Physics 2018-03-06 Jiaji Li , Qian Chen Jiasong Sun , Jialin Zhang , Junyi Ding , Chao Zuo

Optical coherence tomography (OCT) is a non-invasive, micrometer-scale imaging modality that has become a clinical standard in ophthalmology. By raster-scanning the retina, sequential cross-sectional image slices are acquired to generate…

Image and Video Processing · Electrical Eng. & Systems 2024-02-20 Stefan Ploner , Jungeun Won , Julia Schottenhamml , Jessica Girgis , Kenneth Lam , Nadia Waheed , James Fujimoto , Andreas Maier

Optical coherence tomography (OCT) is a label-free, non-invasive 3D imaging tool widely used in both biological research and clinical diagnosis. Current OCT modalities can only visualize specimen tomography without chemical information.…

Optical Diffraction Tomography (ODT) is a powerful non-invasive imaging technique widely used in biological and medical applications. While significant progress has been made in transmission configuration, reflection ODT remains challenging…

Optics · Physics 2026-05-14 Thomas Wasik , Victor Barolle , Alexandre Aubry , Josselin Garnier

In optical diffraction tomography (ODT), the three-dimensional scattering potential of a microscopic object rotating around its center is recovered by a series of illuminations with coherent light. Reconstruction algorithms such as the…

Numerical Analysis · Mathematics 2022-09-02 Robert Beinert , Michael Quellmalz
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