Bond-Selective Full-Field Optical Coherence Tomography
Abstract
Optical coherence tomography (OCT) is a label-free, non-invasive 3D imaging tool widely used in both biological research and clinical diagnosis. Current OCT modalities can only visualize specimen tomography without chemical information. Here, we report a bondselective full-field OCT (BS-FF-OCT), in which a pulsed mid-infrared laser is used to modulate the OCT signal through the photothermal effect, achieving label-free bond-selective 3D sectioned imaging of highly scattering samples. We first demonstrate BS-FF-OCT imaging of 1 {\mu}m PMMA beads embedded in agarose gel. Next, we then show 3D hyperspectral imaging of polypropylene fiber mattress from a standard surgical mask. We then demonstrate BS-FFOCT imaging on biological samples, including cancer cell spheroids and C. elegans. Using an alternative pulse timing configuration, we finally demonstrate the capability of BS-FF-OCT on a bulky and highly scattering 150 {\mu}m thick mouse brain slice.
Cite
@article{arxiv.2301.13355,
title = {Bond-Selective Full-Field Optical Coherence Tomography},
author = {Haonan Zong and Celalettin Yurdakul and Jian Zhao and Zian Wang and Fukai Chen and M. Selim Ünlü and Ji-Xin Cheng},
journal= {arXiv preprint arXiv:2301.13355},
year = {2023}
}