Related papers: Holographic immunoassays
Off-axis X-ray holography is a lensless imaging technique that allows unambiguous retrieval of an object's exit-wave function with high fidelity. It has been used with great success to study nanoscale phenomena and spatio-temporal dynamics…
Colloidal lithography [1] is how patterns are reproduced in a variety of natural systems and is used more and more as an efficient fabrication tool in bio-, opto-, and nano-technology. Nanoparticles in the colloid are made to form a mask on…
Anemia is a prevalent hematological disorder that requires frequent hemoglobin monitoring for early diagnosis and effective management. Conventional hemoglobin assessment relies on invasive blood sampling, limiting its suitability for…
Myoglobin modulates the binding of diatomic molecules to its heme group via hydrogen-bonding and steric interactions with neighboring residues, and is an important benchmark for computational studies of biomolecules. We have performed…
Fast-responding detector arrays are commonly used for imaging rapidly-changing scenes. Besides array detectors, a single-pixel detector combined with a broadband optical spectrum can also be used for rapid imaging by mapping the spectrum…
Recent submillimeter and far-infrared wavelength observations of absorption in the rotational ground-state lines of various simple molecules against distant Galactic continuum sources have opened the possibility of studying the chemistry of…
We propose and demonstrate an experimental method to measure by absorption imaging the size and local column density of a cloud of atoms, even when its smallest dimension is smaller than the resolution of the imaging system. To do this, we…
We present the first results of the holographic beam mapping program for the Canadian Hydrogen Intensity Mapping Experiment (CHIME). We describe the implementation of the holographic technique as adapted for CHIME, and introduce the…
Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based proteomics studies, the sequence coverage greatly depends on…
Colloidal lithography has emerged as a promising alternative to conventional nanofabrication techniques, offering the ability to create nanoscale patterns in a cost-effective and scalable manner. However, it has been so far limited by…
This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40…
Heteropolymer gels can be engineered to release specific molecules into or absorb molecules from a surrounding solution. This remarkable ability is the basis for developing gel applications in extensive areas such as drug delivery, waste…
Proposal to develop an Improved immunological assay employing primary IgG antibodies and secondary IgM antibodies labeled with quantum dots to amplify antigen detection.
Photonic biosensors that use optical resonances to amplify signals from refractive index changes offer high-sensitivity, real-time readout, and scalable, low-cost fabrication. However, when used with classic affinity assays they struggle…
We report on an experimental demonstration of surface acoustic waves monitoring on a thin metal plate with heterodyne optical holography. Narrowband imaging of local optical pathlength modulation is achieved with a frequency-tunable…
In this paper, we investigate the beamforming performances of holographic surfaces implemented as lossless antenna arrays with less than half-wavelength spacing. We first develop a method to quantify the mutual coupling effect among the…
Understanding the binding dynamics of liquid molecules is of fundamental importance in physical and life sciences. However, nanoscale fast dynamics pose great challenges for experimental characterization. Conventionally, the binding…
Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed…
We describe here how to apply the three dimensional imaging technique of refrecative index matched scanning to hydrogel spheres. Hydrogels are water based materials with a low refractive index, which allows for index matching with…
Amphiphilic peptide conjugation affords a significant increase in sensitivity with protein quantification by electrospray-ionization mass spectrometry. This has been demonstrated here for human growth hormone in serum using…