Bond-Selective Intensity Diffraction Tomography
Abstract
Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of ~20 seconds per volume, with a lateral and axial resolution of ~350 nm and ~1.1 micron, respectively. BS-IDT's application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (~100 micron X 100 micron).
Cite
@article{arxiv.2203.13630,
title = {Bond-Selective Intensity Diffraction Tomography},
author = {Jian Zhao and Alex Matlock and Hongbo Zhu and Ziqi Song and Jiabei Zhu and Biao Wang and Fukai Chen and Yuewei Zhan and Zhicong Chen and Yihong Xu and Xingchen Lin and Lei Tian and Ji-Xin Cheng},
journal= {arXiv preprint arXiv:2203.13630},
year = {2023}
}