Related papers: Holographic immunoassays
Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the…
Colloid models have frequently been used to successfully describe the influence of protein-protein interactions on antibody solution properties, but they suffer from inherent problems due to the anisotropic shape of the particles. The net…
The adhesion of cells is mediated by receptors and ligands anchored in apposing membranes. A central question is how to characterize the binding affinity of these membrane-anchored molecules. For soluble molecules, the binding affinity is…
The clinical need for ultra-sensitive molecular analysis has motivated the development of several endpoint assay technologies capable of single molecule readout. These endpoint assays are now primarily limited by the affinity and…
Sparse Aperture Masking (SAM) allows for high-contrast imaging at small inner working angles, however the performance is limited by the small throughput and the number of baselines. We present the concept and first lab results of…
Accurately measuring cell stiffness is challenging due to the invasiveness of traditional methods like atomic force microscopy (AFM) and optical stretching. We introduce a non-invasive off-axis system using holographic imaging and acoustic…
An effective Hamiltonian is considered for hydrogen bonding between two molecules due to the quantum mechanical interaction between the orbitals of the H-atom and the donor and acceptor atoms in the molecules. The Hamiltonian acts on two…
We study the column densities of neutral atomic, molecular, and warm ionized Galactic gas through their continuous absorption of extragalactic X-ray spectra at |b| > 25 degrees. For N(H,21cm) < 5x10^20 cm^-2 there is an extremely tight…
Tight-binding (TB) molecular dynamics (MD) has emerged as a powerful method for investigating the atomic-scale structure of materials --- in particular the interplay between structural and electronic properties --- bridging the gap between…
We present a method for speckle holography that is optimised for crowded fields. Its two key features are an iterativ improvement of the instantaneous Point Spread Functions (PSFs) extracted from each speckle frame and the (optional)…
Publicly available collections of drug-like molecules have grown to comprise 10s of billions of possibilities in recent history due to advances in chemical synthesis. Traditional methods for identifying "hit" molecules from a large…
Measurement of serum growth hormone by mass spectrometry is demonstrated to be unaffected by interferences with growth hormone binding protein as frequently encountered with antibody-based routine test methods and provides an alternative…
We demonstrate a multimode fiber imaging technique based on hydrogel fibers, which are suitable for biomedical applications owing to their biocompatibility and environmental friendliness. High-resolution handwritten images are successfully…
We present an original heterodyne holography method for digital holography that relies on two-dimensional heterodyne detection to record the phase and the amplitude of a f ield. The technique has been tested on objects as much as 13 mm in…
Molecular fingerprinting through absorption spectroscopy is a powerful analytical method. Wide spectral ranges are explored with Doppler-limited resolution. Fast data acquisition, accurate measurements of frequency, intensity, and line…
We investigate the mechanics of two asymmetric ribbons bound at one end and pulled apart at the other ends. We characterize the elastic junction near the bonding and conceptualize it as a bending boundary layer. While the size of this…
In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…
X-ray photoemission and x-ray absorption spectroscopy are important techniques to characterize chemical bonding at surfaces and are often used to identify the strength and nature of adsorbate-substrate interactions. In this study, we judge…
A beam tagging hodoscope prototype made of squared 1 mm$^2$ fibers arranged in two perpendicular planes and coupled to multi-anode photomultipliers has been studied using 65 MeV proton as well as 95 MeV/u $^{12}$C beams at various…
Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when…