Accurately measuring cell stiffness is challenging due to the invasiveness of traditional methods like atomic force microscopy (AFM) and optical stretching. We introduce a non-invasive off-axis system using holographic imaging and acoustic stimulation. This system features an off-axis Mach-Zehnder interferometer and bulk acoustic waves to capture cell mechanics. It employs high-resolution components to create detailed interferograms and allows continuous imaging of cell deformation. Unlike conventional techniques, our method provides high-throughput, label-free measurements while preserving cell integrity. Polyacrylamide beads are tested for high precision, highlighting the potential of the system in early cancer detection, disease monitoring, and mechanobiological research.
@article{arxiv.2407.21182,
title = {Design of a System for Analyzing Cell Mechanics},
author = {Hasan Berkay Abdioglu and Yagmur Isik and Merve Sevgi and Ufuk Gorkem Kirabali and Yunus Emre Mert and Gulnihal Guldogan and Selin Serdarli and Tarik Taha Gulen and Huseyin Uvet},
journal= {arXiv preprint arXiv:2407.21182},
year = {2024}
}