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Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

Point-spread function (PSF) estimation in spatially undersampled images is challenging because large pixels average fine-scale spatial information. This is problematic when fine-resolution details are necessary, as in optimal photometry…

Instrumentation and Methods for Astrophysics · Physics 2021-02-03 Teresa Symons , Michael Zemcov , James Bock , Yun-Ting Cheng , Brendan Crill , Christopher Hirata , Stephanie Venuto

Live cell imaging using metallic nanoparticles as tags is an emerging technique to visualize long and highly dynamic processes due to the lack of photobleaching and high photon rate. However, the lack of excited states as compared to…

Mesoscale and Nanoscale Physics · Physics 2011-01-12 H. E. Grecco , O. E. Martínez

Optical near-fields of small dielectric particles are of particular importance and interests for nanoscale optical engineering such as field localization, fabrication, characterization, sensing and imaging. This paper represents a…

Optics · Physics 2013-04-25 Zengbo Wang , Yi Zhou , Boris Luk'yanchuk

Imaging with a layered superlens is a spatial filtering operation characterized by the point spread function (PSF). We show that in the same optical system the image of a narrow sub-wavelength Gaussian incident field may be surprisingly…

Optics · Physics 2010-04-08 Rafal Kotynski , Tomasz Stefaniuk

Localization microscopy enables imaging with resolutions that surpass the conventional optical diffraction limit. Notably, the MINFLUX method achieves super-resolution by shaping the excitation point-spread function (PSF) to minimize the…

We experimentally demonstrate a non-imaging approach to displacement measurement for complex scattering materials. By spatially controlling the wave front of the light that incidents on the material we concentrate the scattered light in a…

Optics · Physics 2015-06-03 E. G. van Putten , A. Lagendijk , A. P. Mosk

Plasmonic nano-objects have shown great potential in enhancing biological and chemical sensing, light harvesting and energy transfer, and optical and quantum computing to name a few. Therefore, an extensive effort has been vested in…

Nanoscale strain control of exciton funneling is an increasingly critical tool for the scalable production of single photon emitters (SPEs) in two-dimensional materials. However, conventional far-field optical microscopies remain…

Porous nanosponges, percolated with a three-dimensional network of 10-nm sized ligaments, recently emerged as promising substrates for plasmon-enhanced spectroscopy and (photo-)catalysis. Experimental and theoretical work suggests surface…

Mesoscale and Nanoscale Physics · Physics 2018-09-19 Jinhui Zhong , Abbas Chimeh , Anke Korte , Felix Schwarz , Juemin Yi , Dong Wang , Jinxin Zhan , Peter Schaaf , Erich Runge , Christoph Lienau

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

In a previous work we have demonstrated a novel numerical model for the point spread function (PSF) of an optical system that can efficiently model both experimental measurements and lens design simulations of the PSF. The novelty lies in…

Image and Video Processing · Electrical Eng. & Systems 2018-06-25 Matthias Lehmann , Christian Wittpahl , Hatem Ben Zakour , Alexander Braun

In this paper, we show that it is possible to overcome one of the fundamental limitations of super-resolution microscopy techniques: the necessity to be in an \emph{optically homogeneous} environment. Using recent modal approximation…

Analysis of PDEs · Mathematics 2022-07-14 Lorenzo Baldassari , Alice L. Vanel , Pierre Millien

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…

Optics · Physics 2013-09-02 Anthony Barsic , Ginni Grover , Rafael Piestun

The Point Spread Function (PSF) is a key figure of merit for specifying the angular resolution of optical systems and, as the demand for higher and higher angular resolution increases, the problem of surface finishing must be taken…

Instrumentation and Methods for Astrophysics · Physics 2016-10-04 K. Tayabaly , D. Spiga , G. Sironi , R. Canestrari , M. Lavagna , G. Pareschi

A method is described intended for distributed calibration of a probe microscope scanner consisting in a search for a net of local calibration coefficients (LCCs) in the process of automatic measurement of a standard surface, whereby each…

Materials Science · Physics 2015-11-24 Rostislav V. Lapshin

We present the development of a high-precision sample-position-autocorrection system for photoemission experiments. A binocular vision method based on image pattern matching calculations was realized to track the sample position with an…

Superconductivity · Physics 2022-11-03 Shaofeng Duan , Shichong Wang , Yuanyuan Yang , Chaozhi Huang , Lingxiao Gu , Haoran Liu , Wentao Zhang

Plastic nanoparticles present technological opportunities and environmental concerns, but measurement challenges impede product development and hazard assessment. To meet these challenges, we advance a lateral nanoflow assay that integrates…

Soft Condensed Matter · Physics 2022-05-19 Kuo-Tang Liao , Andrew C. Madison , Adam L. Pintar , B. Robert Ilic , Craig R. Copeland , Samuel M. Stavis

The detection of suspended single particles (SSPs) in water is a crucial element for monitoring water ecosystems. Although there are a variety of sensory methods, it is still an unrealized goal to combine multi-wavelength polarized light…

Optics · Physics 2024-10-31 Tong Liu

Light scattering detection in the near field, a rapidly expanding family of scattering techniques, has recently proved to be an appropriate procedure for performing dynamic measurements. Here we report an innovative algorithm, based on the…