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In Real-Time, Feedback-Driven Single Particle Tracking methods, measurements of the emission intensity from a labeled, nanometer-scale particle are used in a feedback loop to track the motion of the particle as it moves inside its native…
Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo…
In astronomy, upcoming space telescopes with wide-field optical instruments have a spatially varying point spread function (PSF). Specific scientific goals require a high-fidelity estimation of the PSF at target positions where no direct…
In fluorescence microscopy, Single Molecule Localization Microscopy (SMLM) techniques aim at localizing with high precision high density fluorescent molecules by stochastically activating and imaging small subsets of blinking emitters.…
We present a versatile setup for investigating the nanofluidic behavior of nanoparticles as a function of the gap distance between two confining surfaces. The setup is designed as an open system which operates with small amounts of…
This Point spread function (PSF) plays a crucial role in many computational imaging applications, such as shape from focus/defocus, depth estimation, and fluorescence microscopy. However, the mathematical model of the defocus process is…
3D-localization of gamma sources has the potential to improve the outcome of radio-guided surgery. The goal of this paper is to analyze the localization accuracy for point-like sources with a single coded aperture camera. We both simulated…
Droplet microfluidics combined with optical detection has become a powerful approach for high-throughput single-cell assays, but these systems often face limited sensitivity and signal heterogeneity due to optical and geometrical…
We demonstrate a method for determining the three-dimensional location of single atoms in a quantum gas microscopy system using a phase-only spatial light modulator to modify the point-spread function of the high-resolution imaging system.…
Accurate astrometry and photometry of saturated and coronagraphic point spread functions (PSFs) are fundamental to both ground- and space-based high contrast imaging projects. For ground-based adaptive optics imaging, differential…
Single quantum emitters coupled to different plasmonic and photonic structures are key elements for integrated quantum technologies. In order to fully exploit these elements, e.g. for quantum enhanced sensors or quantum repeaters, a…
We present the implementation and use of algorithms for matching point-spread functions (PSFs) within the Pan-STARRS Image Processing Pipeline (IPP). PSF-matching is an essential part of the IPP for the detection of supernovae and…
A point spread function (PSF) describes the distribution of light for a pure point source in an astronomical image due to the optics of the instrument. An accurate PSF is key for deconvolution, point source photometry and source removal.…
This paper proposes a robust, high-precision positioning methodology to address localization failures arising from complex background interference in large-scale flight navigation and the computational inefficiency inherent in conventional…
We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solution gives the maximum likelihood estimator of multi-emitter…
Aerosol injectors applied in single-particle diffractive imaging experiments demonstrated their potential in efficiently delivering nanoparticles with high density. Continuous optimization of injector design is crucial for achieving…
Adaptive optics (AO) restore the angular resolution of ground-based telescopes, but at the cost of delivering a time- and space-varying point spread function (PSF) with a complex shape. PSF knowledge is crucial for breaking existing limits…
Deblurring is a fundamental inverse problem in bioimaging. It requires modelling the point spread function (PSF), which captures the optical distortions entailed by the image formation process. The PSF limits the spatial resolution…
Precise measurement of the particle number, spatial distribution and internal state is fundamental to all proposed experiments with ultracold molecules both in bulk gases and optical lattices. Here, we demonstrate in-situ detection of…
The orientation of luminescent molecules in organic light-emitting diodes (OLEDs) strongly influences device performance. However, our understanding of the factors controlling emitter orientation is limited as current measurements only…