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The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. This manuscript reports the development of Polarized Localization Microscopy (PLM),…

Biological Physics · Physics 2017-03-31 Abir Maarouf Kabbani , Christopher V. Kelly

We report a photoluminescence imaging system for locating single quantum emitters with respect to alignment features. Samples are interrogated in a 4~K closed-cycle cryostat by a high numerical aperture (NA=0.9, 100$\times$ magnification)…

Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

The absorption process of an emitter close to a plasmonic antenna is enhanced due to strong local electromagnetic (EM) fields. The emission process, if resonant with the plasmonic system, re-radiates to the far-field by coupling with the…

The first step toward doing high-precision astrometry is the measurement of individual stars in individual images, a step that is fraught with dangers when the images are undersampled. The key to avoiding systematic positional error in…

Astrophysics · Physics 2009-10-05 Jay Anderson , Ivan R. King

Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…

Biological Physics · Physics 2026-04-14 Xiaofeng Zhang , Yongsheng Huang , Jielong Yang , Zhili Wang , Si Chen , Linbo Liu , Xin Ge

Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to…

Optics · Physics 2019-05-22 Oumeng Zhang , Matthew D. Lew

Accurate modelling of the effective point spread function (ePSF) is essential for high-precision photometry and astrometry, particularly in undersampled imaging regimes. In this work, we build on a well-established ePSF modelling framework…

Instrumentation and Methods for Astrophysics · Physics 2025-12-19 Emma Godden , Katherine M. Blundell

Scattering-based single-particle tracking (S-SPT) has opened new avenues for highly sensitive label-free detection and characterization of nanoscopic objects, making it particularly attractive for various analytical applications. However, a…

The accurate modelling of the Point Spread Function (PSF) is of paramount importance in astronomical observations, as it allows for the correction of distortions and blurring caused by the telescope and atmosphere. PSF modelling is crucial…

Instrumentation and Methods for Astrophysics · Physics 2023-09-25 Tobias Liaudat , Jean-Luc Starck , Martin Kilbinger

We describe here a new full 2-D parameterization of the PSFs of the three XMM-Newton EPIC telescopes as a function of instrument, energy, off-axis angle and azimuthal angle, covering the whole field-of-view of the three EPIC detectors. It…

Instrumentation and Methods for Astrophysics · Physics 2015-05-30 A. M. Read , S. R. Rosen , R. D. Saxton , J. Ramirez

Precise control of particle positioning is desirable in many optical propulsion and sorting applications. Here, we develop an integrated platform for particle manipulation consisting of a combined optical nanofiber and optical tweezers…

We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…

Image and Video Processing · Electrical Eng. & Systems 2022-08-05 Bhargav Ghanekar , Vishwanath Saragadam , Dushyant Mehra , Anna-Karin Gustavsson , Aswin Sankaranarayanan , Ashok Veeraraghavan

Unprecedented atomic-scale measurement resolution has recently been demonstrated in single-shot optical localization metrology based on deep-learning analyses of diffraction patterns of topologically structured light scattered from objects.…

Single nanoparticle tracking using optical microscopy is a powerful technique with many applications in biology, chemistry and material sciences. Despite significant advances, localising objects with nanometric position accuracy in a…

Controlling quantum light-matter interactions at scales smaller than the diffraction limit at the single quantum emitter level is a critical challenge to the goal of advancing quantum technologies. We introduce a novel material platform…

By measuring photoelectron tracks, the gas pixel detectors of the Imaging X-ray Polarimetry Explorer satellite provide estimates of the photon detection location and its electric vector position angle (EVPA). However, imperfections in…

Instrumentation and Methods for Astrophysics · Physics 2024-09-24 Jack T. Dinsmore , Roger W. Romani

Single molecule localization microscopy is widely used in biological research for measuring the nanostructures of samples smaller than the diffraction limit. This study uses multifocal plane microscopy and addresses the 3D single molecule…

Computer Vision and Pattern Recognition · Computer Science 2020-01-08 Toshimitsu Aritake , Hideitsu Hino , Shigeyuki Namiki , Daisuke Asanuma , Kenzo Hirose , Noboru Murata

Reliably manufacturing defect free products is still an open challenge for Laser Powder Bed Fusion processes. Particularly, pores that occur frequently have a negative impact on mechanical properties like fatigue performance. Therefore, an…

Computer Vision and Pattern Recognition · Computer Science 2024-08-06 Hans Aoyang Zhou , Jan Theunissen , Marco Kemmerling , Anas Abdelrazeq , Johannes Henrich Schleifenbaum , Robert H. Schmitt