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This industrial Ph.D. project, carried out in collaboration between Radiometer Medical ApS and SDU Centre for Photonics Engineering at the University of Southern Denmark, explored the use of digital holographic microscopy (DHM) for the…
Pinhole imaging is the most primitive and simplest lensless imaging paradigm, capable of transcending the physical limitations of conventional lens optics. This modality is particularly attractive for accessing a virtually infinite depth of…
We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…
Holotomography (HT) has revolutionized quantitative label-free 3D imaging, yet conventional lens-based implementations are fundamentally constrained in field-of-view (FOV) and imaging depth, limiting their utility for critical…
During medicine studies, visualization of certain elements is common and indispensable in order to get more information about the way they work. Currently, we resort to the use of photographs -which are insufficient due to being static- or…
Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…
We present a cost-effective, simple and fast digital holographic microscopy method based upon Rayleigh-Sommerfeld back propagation for identification of the geometrical shape of a cell. The method was tested using synthetic hologram images…
Describe an animal without using the verb look. Can you effectively provide an alternative method for interpreting complex microscopy images while preserving the length scale? The world is filled with features too small for our eyes to see:…
We report the first successful fabrication of three-dimensional models using our fully lensless holographic volumetric additive manufacturing (HoloVAM) platform. In this configuration, tomographic light fields are generated directly from a…
Additive manufacturing using light is commonly constrained by serial voxel-by-voxel or layer-by-layer processing, which fundamentally limits fabrication speed and scalability. Here, we introduce a single-exposure holographic…
HiLo microscopy is a powerful, low-cost, and easily configurable technique for acquiring high-contrast optically-sectioned images. However, traditional HiLo microscopes are based on coherent light sources with diffusive glass plates or…
Optical imaging technologies are central to discovery in the life and physical sciences, yet their impact depends on how readily they can be built, adapted, and sustained across laboratories. Digital fabrication, including desktop 3D…
Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…
This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…
Hair-thin optical fiber endoscopes have opened up new paradigms for advanced imaging applications in vivo. In certain applications, such as optical coherence tomography (OCT), light-shaping structures may be required on fiber facets to…
The democratization of fabrication equipment has spurred recent interest in maskless grayscale lithography for both 2D and 3D microfabrication. However, the design of suitable template images remains a challenge. This work presents a…
Ultrasound imaging is developing an open-source ecosystem, especially for software frameworks. Little exists on the open-hardware side. Hence a focus was put on producing easy-to-use technological (hardware and software) kit to allow anyone…
One-photon or two photon absorption by dye molecules in photopolymers enable direct 2D & 3D lithography of micro/nano structures with high spatial resolution and can be used effectively in fabricating artificially structured nanomaterials.…
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…
One of the most common methods for reconstructing three-dimensional (3D) images of real objects is digital holography. This technique relies on the use of electro-optical devices that modify the phase or amplitude of light fields in a…