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We present a maskless microscope projection lithography system (MPLS), in which photomasks have been replaced by a Digital Micromirror Device type spatial light modulator (DMD, Texas Instruments). Employing video projector technology high…

Quantitative Methods · Quantitative Biology 2009-11-13 Thomas Naiser , Timo Mai , Wolfgang Michel , Albrecht Ott

Minimally invasive imaging with fiber optic endoscopes is crucial for in vivo visualization of tissue morphology, particularly because visualizing structural changes in tissue can support applications such as the early diagnosis of tumors.…

A powerful and flexible method of 3D nano-printing, based on focusing charged aerosol, has been developed. The self-consistent electric field configuration, created with a holey floating mask and used as the scaffold for printing…

Applied Physics · Physics 2018-12-14 Wooik Jung , Yoon-ho Jung , Peter V. Pikhitsa , Jooyeon Shin , Kijoon Bang , Jicheng Feng , Mansoo Choi

Lensless digital holographic microscopy (LDHM) offers very large field-of-view label-free imaging crucial, e.g., in high-throughput particle tracking and biomedical examination of cells and tissues. Compact layouts promote point-of-case and…

Optics · Physics 2023-10-24 Piotr Arcab , Mikolaj Rogalski , Maciej Trusiak

The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and…

We demonstrate a compact and easy-to-build computational camera for single-shot 3D imaging. Our lensless system consists solely of a diffuser placed in front of a standard image sensor. Every point within the volumetric field-of-view…

Computer Vision and Pattern Recognition · Computer Science 2017-10-06 Nick Antipa , Grace Kuo , Reinhard Heckel , Ben Mildenhall , Emrah Bostan , Ren Ng , Laura Waller

We present a comprehensive guide to light-sheet microscopy (LSM) to assist scientists in navigating the practical implementation of this microscopy technique. Emphasizing the applicability of LSM to image both static microscale and…

Integration of complex photonic structures onto optical fiber facets enables powerful platforms with unprecedented optical functionalities. Conventional nanofabrication technologies, however, do not permit viable integration of complex…

Polarization microscopy provides insights into the structure and orientational organization of biomolecules and their architectures in cells. The above key functional signatures, which are natively 3D, can be only detected in 2D for a…

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

A light field print (LFP) displays three-dimensional (3D) information to the naked-eye observer under ambient white light illumination. Changing perspectives of a 3D image are seen by the observer from varying angles. However, LFPs appear…

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

Image and Video Processing · Electrical Eng. & Systems 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

Advancements in high-throughput biomedical applications require real-time, large field-of-view (FOV) imaging. While current 2D lens-free imaging (LFI) systems improve FOV, they are often hindered by time-consuming multi-position…

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

Microfluidics have emerged as a vital tool in various scientific and engineering applications, ranging from biomedical diagnostics to chemical analysis. However, conventional fabrication methods often necessitate costly equipment and…

Instrumentation and Detectors · Physics 2023-08-07 Habilou Ouro-Koura , Ayobami Ogunmolasuyi , Othman Suleiman , Kausik S Das

The future success of integrated circuits (IC) technology relies on the continuing miniaturization of the feature size, allowing more components per chip and higher speed. Extreme anisotropy opens new opportunities for spatial pattern…

Optics · Physics 2016-08-10 Jingbo Sun , Tianboyu Xu , Natalia M. Litchinitser

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

The emerging new paradigm of technologies, the internet of things, entails a process of device miniaturization to combine several functional components, such as sensors, actuators, and powering elements, in a single individual on-chip…

Recently introduced, spaceplates achieve the propagation of light for a distance greater than their thickness. In this way, they compress optical space, reducing the required distance between optical elements in an imaging system. Here we…

Optics · Physics 2023-06-01 Nicholas J. Sorensen , Michael T. Weil , Jeff S. Lundeen

Metasurface-generated holography has emerged as a promising route for fully reproducing vivid scenes by manipulating the optical properties of light using ultra-compact devices. However, achieving multiple holographic images using a single…

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