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A simple method of constructing the 3D surface of non-transparent micro-objects by extending the depth-of-field on the whole attainable surface is presented. The series of images of a sample are recorded by the sequential movement of the…

Instrumentation and Detectors · Physics 2009-04-15 V. V. Berejnov

We present an educational tool, a microscope with a video camera, that can be fabricated either from a standard microscope or assembled from inexpensive, commercially available components (objectives, beam splitters, LEDs, linear stages)…

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

Fiber imaging bundles are widely used as thin, passive image conduits for miniaturised and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase…

Optics · Physics 2020-10-28 Michael R Hughes

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

We report a compact, cost-effective and field-portable lensless imaging platform for quantitative microscopy. In this platform, the object is placed on top of an image sensor chip without using any lens. We use a low-cost galvo scanner to…

Image and Video Processing · Electrical Eng. & Systems 2019-04-26 He Zhang , Zichao Bian , Shaowei Jiang , Jian Liu , Pengming Song , Guoan Zheng

Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…

Image and Video Processing · Electrical Eng. & Systems 2020-04-20 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Lensless digital holographic microscopy (LDHM) relies on interference between an unscattered reference wave and a weakly scattered object wave - an assumption that rapidly fails in turbid samples under multiple scattering. To overcome this…

A fundamental challenge in endoscopy is how to fabricate a small fiber-optic probe that can achieve comparable function to probes with large, complicated optics (e.g., high resolution and extended depth of focus). To achieve high resolution…

Optical imaging through scattering media is an important challenge in a variety of fields ranging from microscopy to autonomous vehicles. While advanced wavefront shaping techniques have offered significant breakthroughs in the past decade,…

Optics · Physics 2023-05-23 Omri Haim , Jeremy Boger-Lombard , Ori Katz

Microscopy plays a crucial role in hematology and diagnosis of infectious diseases worldwide. For malaria alone, more than 200 million slides are read by manual microscopists every year. High quality thin blood smears are essential for…

Medical Physics · Physics 2023-04-21 Jerome Nowak , Anesta Kothari , Hongquan Li , Jaspreet Pannu , Dani Algazi , Manu Prakash

Recent development of lensless imagers has enabled three-dimensional (3D) imaging through a thin piece of optics in close proximity to a camera sensor. A general challenge of wide-field lensless imaging is the high computational complexity…

Optics · Physics 2021-08-17 Feng Tian , Junjie Hu , Weijian Yang

Silicon is an excellent material for microelectronics and integrated photonics with untapped potential for mid-IR optics. Despite broad recognition of the importance of the third dimension, current lithography methods do not allow…

3D printing allows rapid fabrication of complex objects from digital designs. One 3D-printing process, direct laser writing, polymerises a light-sensitive material by steering a focused laser beam through the shape of the object to be…

Metasurface holography, the reconstruction of holographic images by modulating the spatial amplitude and phase of light using metasurfaces, has emerged as a next-generation display technology. However, conventional fabrication techniques…

Exposure to bio-aerosols such as mold spores and pollen can lead to adverse health effects. There is a need for a portable and cost-effective device for long-term monitoring and quantification of various bio-aerosols. To address this need,…

Applied Physics · Physics 2022-11-23 Yi Luo , Yijie Zhang , Tairan Liu , Alan Yu , Yichen Wu , Aydogan Ozcan

Chapter 1 gives a brief history of the field of holography along with an overview of this thesis. A more detailed description of holography is provided in Chapter 2 along with a discussion of digital holography. Chapter 3 examines the…

Optics · Physics 2009-09-30 John Tapsell

On-chip optical architectures that enable angle-resolved spectroscopy are essential for advancing photonic platforms towards low-volume, scalable, and cryo-compatible devices. Here, we introduce spatially resolved momentum-space imaging…

Optical microscopy has a diffraction limited resolution of about 250 nm. Fluorescence methods (e.g. PALM, STORM, STED) beat this, but they are still limited to 10 s of nm, and the images are an indirect pointillist representation of only…