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Holography exploits the interference of light fields to obtain a systematic reconstruction of the light fields wavefronts. Classical holography techniques have been very successful in diverse areas such as microscopy, manufacturing…

The visualization of pure phase objects by wavefront sensing has important applications ranging from surface profiling to biomedical microscopy, and generally requires bulky and complicated setups involving optical spatial filtering,…

Optics · Physics 2023-08-29 Jianing Liu , Hao Wang , Yuyu Li , Lei Tian , Roberto Paiella

We present an algorithm for coherent diffractive imaging with phaseless measurements. It treats the forward model as a combination of coherent and incoherent waves. The algorithm reconstructs absorption and phase contrast that quantifies…

Computational Physics · Physics 2022-08-25 Miguel Moscoso , Alexei Novikov , George Papanicolaou , Chrysoula Tsogka

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

We propose an holographic microscopy reconstruction method, which propagates the hologram, in the object half space, in the vicinity of the object. The calibration yields reconstructions with an undistorted reconstruction grid i.e. with…

Optics · Physics 2015-06-16 Nicolas Verrier , Danier Alexandre , Gilles Tessier , Michel Gross

We report a demonstration of phase-resolved vibrometry, in which out-of-plane sinusoidal motion is assessed by heterodyne holography. In heterodyne holography, the beam in the reference channel is an optical local oscillator (LO). It is…

Optics · Physics 2015-06-12 Nicolas Verrier , Michel Gross , Michael Atlan

A single intensity-only holographic interferogram can records the full amplitude and phase information of optical field. However, current digital holography technologies cannot recover the lossless phase information from a single…

Image and Video Processing · Electrical Eng. & Systems 2020-03-09 Qinnan Zhang , Shengyu Lu , Jiaosheng Li , Wenjie Li , Dong Li , Xiaoxu Lu , Liyun Zhong , Jindong Tian

Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However,…

Quantitative phase imaging (QPI) through multi-core fibers (MCFs) has been an emerging in vivo label-free endoscopic imaging modality with minimal invasiveness. However, the computational demands of conventional iterative phase retrieval…

We present a dual-wavelength external holographic microscopy module for quantitative phase imaging of 3D structures with extended thickness range. This is done by simultaneous acquisition of two off-axis interferograms, each of which at a…

Optics · Physics 2019-04-10 Nir A. Turko , Natan T. Shaked

We present an image recovery approach to improve amplitude and phase reconstruction from single shot digital holograms, using iterative reconstruction with alternating updates. This approach allows the flexibility to apply different priors…

Optics · Physics 2016-10-04 Dennis J. Lee , Charles A. Bouman , Andrew M. Weiner

Computational imaging modalities support a simplification of the active architectures required in an imaging system and these approaches have been validated across the electromagnetic spectrum. Recent implementations have utilized…

Classical Physics · Physics 2016-08-24 Thomas Fromenteze , Xiaojun Liu , Michael Boyarsky , Jonah Gollub , David R. Smith

We present a single-image numerical phase retrieval method for Zernike phase-contrast microscopy (ZPM) that addresses halo and shade-off artifacts, as well as the weak phase condition, without requiring hardware modifications. By employing…

Forming a desired optical field distribution from a given source requires precise spatial control of a field's amplitude and phase. Low-loss metasurfaces that allow extreme phase and polarization control of optical fields have been…

Optics · Physics 2021-05-26 Brian O. Raeker , Anthony Grbic

Quantum phase imaging enables the analysis of transparent samples with thickness and refractive index variations in scenarios requiring precise measurements under low-light conditions. Here, we present a compact quantum phase-gradient…

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…

Optics · Physics 2025-01-17 David Alonso , Javier Garcia , Vicente Mico

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Polarisation holography generally demands polarisation-sensitive holograms for reconstructing either polarisation-multiplexed holographic images or polarisation-sensitive image channels. To date, polarisation holography is underpinned by…

Optics · Physics 2022-07-22 Haoran Ren

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller
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