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We present a powerful and cost-effective method for active illumination using a digital micromirror device (DMD) for quantitative phase imaging techniques. Displaying binary illumination patterns on a DMD with appropriate spatial filtering,…

Optics · Physics 2023-07-19 Seungwoo Shin , Kyoohyun Kim , Jonghee Yoon , YongKeun Park

We present an overview of the performances of a plenoptic microscope which combines the high sensitivity of a laser optical feedback imaging setup , the high resolution of optical synthetic aperture and a shot noise limited signal to noise…

Optics · Physics 2015-06-19 W Glastre , O Hugon , O Jacquin , H Guillet de Chatellus , E Lacot

Digital holography (DH) is a promising method for non-contact surface topography because the reconstructed phase image can visualize the nanometer unevenness in a sample. However, the axial range of this method is limited to the range of…

A low-cost device for registration-free quantitative phase microscopy (QPM) based on the transport of intensity equation (TIE) of cells in continuous flow is presented. The method uses acoustic focusing to align cells into a single plane…

High harmonic generation in the interaction of femtosecond lasers with atoms and molecules opens the path to molecular orbital tomography and to probe the electronic dynamics with attosecond-{\AA}ngstr\"{o}m resolutions. Molecular orbital…

Based on phase retrieval, lensless coherent imaging and in particular holography offers quantitative phase and amplitude images. This is of particular importance for spectral ranges where suitable lenses are challenging, such as for hard…

Image and Video Processing · Electrical Eng. & Systems 2022-09-01 Simon Huhn , Leon Merten Lohse , Jens Lucht , Tim Salditt

An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop…

Optics · Physics 2025-05-06 Keiichiro Toda , Miu Tamamitsu , Ryoichi Horisaki , Takuro Ideguchi

The quantum noise of light fundamentally limits optical phase sensors. A semiclassical picture attributes this noise to the random arrival time of photons from a coherent light source such as a laser. An engineered source of squeezed states…

We propose a holographic microscopy method based on single-pixel imaging technology (HM-SPI). We used a holographic microscopy method based on in-line Gabor holography. In single-pixel imaging technology, cyclic binary masks and…

Optics · Physics 2025-04-11 Arthur G. Fedorov

Experiments with networks of optogenetically altered neurons require stimulation with high spatio-temporal selectivity. Computer-assisted holography is an energy-efficient method for robust and reliable addressing of single neurons on the…

Optimization and Control · Mathematics 2013-02-04 Stephan C. Kramer , Johannes Hagemann , D. Russell Luke

We show that de-focused single particle images recorded using a cryo-electron microscope (cryoEM) system may be processed like a Fresnel zone in-line hologram to obtain physically meaningful quantitative phase maps associated with…

Image and Video Processing · Electrical Eng. & Systems 2021-11-24 Apoorv Pant , Manidipa Banerjee , Kedar Khare

Digital holography is an interference-based imaging technique capable of recording both the amplitude and phase of an electromagnetic field. It can be obtained at the laser illumination wavelength, but also with second-harmonic generation,…

We propose a new gradient method for holography, where a phase-only hologram is parameterized by not only the phase but also amplitude. The key idea of our approach is the formulation of a phase-only hologram using an auxiliary amplitude.…

Information Theory · Computer Science 2024-05-22 Ittetsu Uchiyama , Chihiro Tsutake , Keita Takahashi , Toshiaki Fujii

Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…

Optics · Physics 2023-08-04 Herve Hugonnet , HyunJun Han , Weisun Park , YongKeun Park

Large field-of-view (FOV) microscopic imaging with high lateral resolution (1-2 microns for high space-bandwidth product) plays a pivotal role in biomedicine and biophotonics, especially within the label-free regime, e.g., for whole slide…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

A method is described for the reconstruction of the amplitude and phase of the object exit wave function by phase-plate transmission electron microscopy. The proposed method can be considered as in-line holography and requires three images,…

Materials Science · Physics 2017-05-24 B. Gamm , M. Dries , K. Schultheiss , H. Blank , A. Rosenauer , R. R. Schröder , D. Gerthsen

We extend our previously proposed image reconstruction method, which allows confocal microscopes to capture periodically moving objects at frequencies beyond their frame rates, to three-dimensional and two-dimensional wide-field imaging.…

Optics · Physics 2026-03-03 Kanta Adachi , Soyoka Hemmi , Nobutomo Nakamura

Phase retrieval in inline holography is a fundamental yet ill-posed inverse problem due to the nonlinear coupling between amplitude and phase in coherent imaging. We present a novel off-the-shelf solution that leverages a diffusion model…

Optics · Physics 2025-11-12 Jeongsol Kim , Chanseok Lee , Jongin You , Jong Chul Ye , Mooseok Jang