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While characterization of coherent wavefields is essential to laser, x-ray and electron imaging, sensors measure the squared magnitude of the field, rather than the field itself. Holography or phase retrieval must be used to characterize…

Image and Video Processing · Electrical Eng. & Systems 2020-12-10 David J. Brady , Timothy J. Schulz , Chengyu Wang

We demonstrate high-resolution non-iterative holographic coherent diffraction imaging with hard X-rays using a novel phase-shifting reference, fabricated by atomic layer deposition to produce nanosharp 3D structure. The method surpasses the…

Mesoscale and Nanoscale Physics · Physics 2016-10-17 Mirna Saliba , Jeroen Bosgra , Christoph Rau , Christian David , Aaron D. Parsons , Ulrich H. Wagner , Pierre Thibault

We present a new digital holographic microscope (DHM) with a low coherent source for the quantitative imaging of smooth and optically rough objects. The experimental design of the microscope uses an in-line experimental geometry based on…

Quantitative phase imaging (QPI) recovers the exact wavefront of light from the intensity measured by a camera. Topographical maps of translucent microscopic bodies can be extracted from these quantified phase shifts. We demonstrate…

In both light optics and electron optics, the amplitude of a wave scattered by an object is an observable that is usually recorded in the form of an intensity distribution in a real space image or a diffraction image. In contrast, retrieval…

We demonstrate two-step phase-shifting interferometry (holography) of complex laser modes generated by a spatial light modulator (SLM), in which the amplitude and phase of the signal are determined directly from measurements of…

Optics · Physics 2024-09-16 Lark E. Bradsby , Andrew A. Voitiv , Mark E. Siemens

Phase retrieval is the inverse problem of recovering a signal from magnitude-only Fourier measurements, and underlies numerous imaging modalities, such as Coherent Diffraction Imaging (CDI). A variant of this setup, known as holography,…

Machine Learning · Computer Science 2021-04-22 Hannah Lawrence , David A. Barmherzig , Henry Li , Michael Eickenberg , Marylou Gabrié

Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…

Analysis of PDEs · Mathematics 2026-05-29 Thorsten Hohage , Milad Karimi , Björn Müller

Quantitative phase imaging (QPI) is a valuable label-free modality that has gained significant interest due to its wide potentials, from basic biology to clinical applications. Most existing QPI systems measure microscopic objects via…

Optics · Physics 2021-12-22 Chenfei Hu , Mikhail E. Kandel , Young Jae Lee , Gabriel Popescu

Second-harmonic generation (SHG) microscopy provides label-free imaging of biological tissues with unique contrast mechanisms, but its resolution is limited by the diffraction limit. Here, we present the first experimental demonstration of…

Optics · Physics 2023-04-28 Jungho Moon , Sungsam Kang , Jin Hee Hong , Seokchan Yoon , Wonshik Choi

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

Optics · Physics 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

Image and Video Processing · Electrical Eng. & Systems 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

In this work, we present a highly efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination based on traditional bright-field microscope. As a new type of coded illumination, the LED array provides a…

Optics · Physics 2017-07-14 Jiaji Li , Qian Chen , Jialin Zhang , Yan Zhang , Linpeng Lu , Chao Zuo

Imaging through dynamic scattering media, such as biological tissue, presents a fundamental challenge due to light scattering and the formation of speckle patterns. These patterns not only degrade image quality but also decorrelate rapidly,…

X-ray near field holography has proven to be a powerful 2D and 3D imaging technique with applications ranging from biomedical research to material sciences. To reconstruct meaningful and quantitative images from the measurement intensities,…

Image and Video Processing · Electrical Eng. & Systems 2024-03-04 Jens Lucht , Leon M. Lohse , Thorsten Hohage , Tim Salditt

This paper reported a general noninterferometric high-accuracy quantitative phase imaging (QPI) method for arbitrary complex valued objects. Given by a typical 4f optical configuration as the imaging system, three frames of small-window…

Image and Video Processing · Electrical Eng. & Systems 2021-05-05 Jianhui Huang , An Pan , Huiliang Jin , Guoxiang Meng , Qian Ye

Metasurfaces are optically thin metamaterials that promise complete control of the wavefront of light but are primarily used to control only the phase of light. Here, we present an approach, simple in concept and in practice, that uses…

Coherent Diffractive Imaging is a lensless technique that allows imaging of matter at a spatial resolution not limited by lens aberrations. This technique exploits the measured diffraction pattern of a coherent beam scattered by periodic…

Computational Physics · Physics 2025-08-27 Alessandro Colombo , Davide Emilio Galli , Liberato De Caro , Francesco Scattarella , Elvio Carlino

Entanglement is a key resource in quantum technologies, enhancing precision and resolution in imaging and sensing by leveraging the cross-correlation of photon pairs. This correlation enables precise time synchronization of photons reaching…