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Phase-contrast microscopy converts the optical phase introduced by transparent, unlabeled specimens into modulation in the intensity image. Modern phase imaging techniques are capable of quantifying phase shift at each point in the field of…

Confocal imaging and phase imaging are powerful tools in life science research and industrial inspection. To coherently link the two techniques with different depth resolutions, we introduce an optical frequency comb (OFC) to microscopy.…

Imaging transparent samples remains an ongoing challenge in the study of unstained biological cells and material samples. Widely used methods trade off system complexity, cost and bulk, computational efficiency and information content. Here…

Quantum imaging with undetected light (QIUL) can retrieve amplitude and phase information of an object by exploiting the quantum correlations of photon-pairs generated through spontaneous parametric down conversion (SPDC), where the…

Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. Here we demonstrate…

Image and Video Processing · Electrical Eng. & Systems 2021-05-28 Luzhe Huang , Tairan Liu , Xilin Yang , Yi Luo , Yair Rivenson , Aydogan Ozcan

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

Label-free quantitative phase imaging is a vital tool for optical microscopy and metrology applications. A hair-thin multimode fiber stands out as a very attractive platform for minimally invasive imaging. Here we propose and experimentally…

Detection of phase variations across optically transparent samples is often a difficult task. We propose and demonstrate a compact, lightweight and low cost quantitative phase contrast imager. Light diffracted from a pinhole is incident on…

We present a new four-dimensional phase unwrapping approach for time-lapse quantitative phase microscopy, which allows reconstruction of optically thick objects that are optically thin in a certain temporal point and angular view. We thus…

Optics · Physics 2019-09-04 Gili Dardikman , Gyanendra Singh , Natan T. Shaked

We present an entanglement-based quantitative phase gradient microscopy technique that employs principles from quantum ghost imaging and ghost diffraction. In this method, a transparent sample is illuminated by both photons of an entangled…

Quantum Physics · Physics 2026-03-09 Yingwen Zhang , Paul-Antoine Moreau , Duncan England , Ebrahim Karimi , Benjamin Sussman

New lensless diffractive X-ray technic for micro-scale imaging of biological tissue is based on quantitative phase retrieval schemes. By incorporating refraction, this method yields improved contrast compared to purely absorption-based…

Numerical Analysis · Mathematics 2018-03-28 Victor Palamodov

Phase and polarisation of coherent light are highly perturbed by interaction with microstructural changes in pre-malignant tissue, holding promise for label-free early cancer detection in endoscopically accessible tissues such as the…

In the last five decades, iterative phase retrieval methods draw large amount of interest across the research community as a non-interferometric approach to recover quantitative phase distributions from one (or more) intensity measurement.…

Optics · Physics 2020-07-21 Nathaniel Hai , Joseph Rosen

Fourier ptychographic microscopy (FPM) is a recently developed imaging modality that uses angularly varying illumination to extend a system performance beyond the limit defined by its optical elements. The FPM technique applies a novel…

Optics · Physics 2014-05-02 Xiaoze Ou , Roarke Horstmeyer , Changhuei Yang , Guoan Zheng

Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…

Image and Video Processing · Electrical Eng. & Systems 2020-04-20 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

We present a reconstruction technique for simultaneous retrieval of absorption and phase shifting properties of an object recorded by in-line holography. The routine is experimentally tested by applying it to optical holograms of a pure…

Optics · Physics 2010-10-28 Tatiana Latychevskaia , Hans-Werner Fink

Phase imaging techniques extract the optical path-length information of a scene, whereas wavefront sensors provide the shape of an optical wavefront. Since these two applications have different technical requirements, they have developed…

Optics · Physics 2018-02-28 F. Soldevila , V. Durán , P. Clemente , J. Lancis , E. Tajahuerce

Minimally invasive imaging with fiber optic endoscopes is crucial for in vivo visualization of tissue morphology, particularly because visualizing structural changes in tissue can support applications such as the early diagnosis of tumors.…

Addition of random phase to the object light is required in computer-generated holograms (CGHs) to widely diffuse the object light and to avoid its concentration on the CGH; however, this addition causes considerable speckle noise in the…

Optics · Physics 2015-06-24 Tomoyoshi Shimobaba , Tomoyoshi Ito