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In this paper we present a method to robustly evaluate the quantitative accuracy of various tomographic phase microscopy (TPM) methods with a multiple scattering 3D-printed microphantom with known geometry and refractive index distribution.…
We develop a novel algorithm for large-scale holographic reconstruction of 3D particle fields. Our method is based on a multiple-scattering beam propagation method (BPM) combined with sparse regularization that enables recovering dense 3D…
Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation…
Magnetic Resonance Imaging (MRI) is a powerful imaging technique widely used for visualizing structures within the human body and in other fields such as plant sciences. However, there is a demand to develop fast 3D-MRI reconstruction…
Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…
Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…
Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…
Simultaneous multislice (SMS) imaging is a one of the acceleration technique of magnetic resonance imaging. SMS requires accurate sensitivity distributions in the slice plane for each receiving coil. This requirement is difficult to satisfy…
Over the past decade, reflection matrix microscopy (RMM) and advanced image reconstruction algorithms have emerged to address the fundamental imaging depth limitations of optical microscopy in thick biological tissues and complex media. In…
The main disadvantage of Magnetic Resonance Imaging (MRI) are its long scan times and, in consequence, its sensitivity to motion. Exploiting the complementary information from multiple receive coils, parallel imaging is able to recover…
In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…
High-resolution lithographic techniques are often limited by low volumetric throughput, since there is no universal and scalable manufacturing process that can produce 3D metasurfaces. In this work, we demonstrate a high-speed holographic…
Three-dimensional ultrasound enables real-time volumetric visualization of anatomical structures. Unlike traditional 2D ultrasound, 3D imaging reduces reliance on precise probe orientation, potentially making ultrasound more accessible to…
Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…
Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…
Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…
Snapshot multi-dimensional imaging offers a promising alternative to traditional low-dimensional imaging techniques by enabling the simultaneous capture of spatial, spectral, polarization, and other information in a single shot for improved…
X-ray time-resolved tomography is one of the most popular X-ray techniques to probe dynamics in three dimensions (3D). Recent developments in time-resolved tomography opened the possibility of recording kilohertz-rate 3D movies. However,…
Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…
Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…