Related papers: Multi-purpose SLM-light-sheet microscope
We demonstrate the combination of a hemispherical solid immersion lens with a micro-photoluminescence setup. Two advantages introduced by the SIL, an improved resolution of 0.4 times the wavelength in vacuum and a 5 times enhancement of the…
A spatial light modulator (SLM) and a pair of galvanometer-mounted mirrors (GMM) were combined into an optical tweezers set-up. This provides great flexibility as the SLM creates an array of traps which can be moved smoothly and quickly…
Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multi-color capability with high-quality illumination is currently lacking.…
The lensless endoscope (LE) is a promising device to acquire in vivo images at a cellular scale. The tiny size of the probe enables a deep exploration of the tissues. Lensless endoscopy with a multicore fiber (MCF) commonly uses a spatial…
We propose and experimentally demonstrate a differential microscopy method to obtain simultaneous amplitude, phase, and quantitative polarization gradient imaging in a single experimental embodiment. A full-field optical spatial…
Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…
Additive manufacturing techniques enable the fabrication of functional microstructures with mechanical and chemical properties tailored to their intended use. One common technique is stereolithography, which has recently been augmented in…
We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable electro-optical spatial light modulator in an intermediate image plane enables…
We report the first successful fabrication of three-dimensional models using our fully lensless holographic volumetric additive manufacturing (HoloVAM) platform. In this configuration, tomographic light fields are generated directly from a…
We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet…
We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…
Holographic light potentials generated by phase-modulating liquid-crystal spatial light modulators (SLMs) are widely used in quantum technology applications. Accurate calibration of the wavefront and intensity profile of the laser beam at…
Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…
Active metasurfaces enable dynamic control of light for applications in beam steering, pixelated holography, and adaptive optics, but demonstrations of two-dimensional (2D) electrically addressable arrays have so far been limited. Here we…
Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…
Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…
Selective plane illumination microscopy (SPIM), or light sheet, is a powerful three-dimensional imaging approach. However, access to and interfacing microscopes with microfluidics have remained challenging. Complex interfacing with…
Line Intensity Mapping (LIM) is a new observational technique that uses low-resolution observations of line emission to efficiently trace the large-scale structure of the Universe out to high redshift. Common mm/sub-mm emission lines are…
Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…
Adaptive optics enables the deployment of interferometer-based spectroscopy without the need for moving parts necessary for scanning the interferometer arms. Here, we employ a Michelson Interferometer in conjunction with a Spatial Light…