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Two-photon (2P) microscopy is a powerful technique for deep-tissue fluorescence imaging; however, tissue scattering limits its effectiveness for depth imaging using conventional approaches. Despite typical strategies having been put forward…

Optics · Physics 2025-05-21 Lei Zhu , Bernhard Rauer , Hilton B. De Aguiar , Sylvain Gigan

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…

Speckle-correlation imaging techniques are widely used for non-invasive imaging through complex scattering media. While light propagation through multimode fibers and scattering media share many analogies, reconstructing images through…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

On-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and…

We exploit memory effect speckle correlations for the imaging of incoherent linear (single-photon) fluorescent sources behind scattering tissue. While memory effect-based imaging techniques have been heavily studied in the past, for thick…

There are several methods to focus light behind a scattering medium, but very few use fluorescence light as feedback or can be used without access to the distal side of the scatterer. Among all the wave-front shaping techniques, retrieving…

Optics · Physics 2020-03-18 Evolene Premillieu , Rafael Piestun

Recently, fluorescence-based optical techniques have emerged as a powerful tool to probe information in the mammalian brain. However, tissue heterogeneities prevent clear imaging of deep neuron bodies due to light scattering. While several…

Optically focusing and imaging through strongly scattering media are challenging tasks but have widespread applications from scientific research to biomedical applications and daily life. Benefiting from the memory effect (ME) for speckle…

Optics · Physics 2021-09-17 Sujit Kumar Sahoo , Dongliang Tang , Cuong Dang

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Wavefront shaping is a powerful method to refocus light through a scattering medium. Its application to large spectral bandwidths or multiple wavelengths refocusing for nonlinear bio-imaging in-depth is however limited by spectral…

Imaging through scattering media is a longstanding issue in a wide range of applications, including biomedicine, security, and astronomy. Speckle-correlation imaging is promising for non-invasively seeing through scattering media by…

Optics · Physics 2022-10-13 Yuto Endo , Jun Tanida , Makoto Naruse , Ryoichi Horisaki

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

Imaging with optical resolution through highly scattering media is a long sought-after goal with important applications in deep tissue imaging. Although being the focus of numerous works, this goal was considered impractical until recently.…

Optics · Physics 2012-02-10 Ori Katz , Eran Small , Yaron Silberberg

Optical imaging deep inside scattering media remains a fundamental problem in bio-imaging. While wavefront shaping has been shown to allow focusing of coherent light at depth, achieving it non-invasively remains a challenge. Various…

Optics · Physics 2019-12-24 Antoine Boniface , Baptiste Blochet , Jonathan Dong , Sylvain Gigan

Imaging through scattering media is encountered in many disciplines or sciences, ranging from biology, mesescopic physics and astronomy. But it is still a big challenge because light suffers from multiple scattering is such media and can be…

Neural and Evolutionary Computing · Computer Science 2017-08-29 Meng Lyu , Hao Wang , Guowei Li , Guohai Situ

Achieving high spatial resolution is the goal of many imaging systems. Designing a high-resolution lens with diffraction-limited performance over a large field of view remains a difficult task in imaging system design. On the other hand,…

Recently introduced angular-memory-effect based techniques enable non-invasive imaging of objects hidden behind thin scattering layers. However, both the speckle-correlation and the bispectrum analysis are based on the statistical average…

Optics · Physics 2017-11-22 Tengfei Wu , Jonathan Dong , Xiaopeng Shao , Sylvain Gigan
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