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Optical coherence tomography (OCT) has seen widespread success as an in vivo clinical diagnostic 3D imaging modality, impacting areas including ophthalmology, cardiology, and gastroenterology. Despite its many advantages, such as high…

Spectroscopy is the basic tool for studying molecular physics and realizing bio-chemical sensing. However, it is challenging to realize sub-femtometer resolution spectroscopy over broad bandwidth. In this paper, broadband and…

Optics · Physics 2022-12-19 Bingxin Xu , Yangyang Wan , Xinyu Fan , Zuyuan He

Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation…

Hyperspectral optoacoustic microscopy (OAM) enables obtaining images with label-free biomolecular contrast, offering excellent perspectives as a diagnostic tool to assess freshly excised and unprocessed tissues. However, time-consuming…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Constantin Berger , Myeongseop Kim , Lukas Scheel-Platz , Vasilis Ntziachristos , Dominik Jüstel , Miguel A. Pleitez

We report on a technically simple approach to achieve high-resolution and high-sensitivity Fourier-domain OCT imaging in the mid-infrared range. The proposed OCT system employs an InF3 supercontinuum source. A specially designed dispersive…

Optics · Physics 2021-09-15 Ivan Zorin , Paul Gattinger , Andrii Prylepa , Bettina Heise

Optical coherence tomography (OCT) is a high-resolution three-dimensional imaging technique that enables non-destructive measurements of surface and subsurface microstructures. Recent developments of OCT operating in the mid-infrared (MIR)…

Optics · Physics 2020-07-15 Ivan Zorin , Rong Su , Bettina Heise , Bernhard Lendl , Markus Brandstetter

Lensless digital holographic microscopy (LDHM) relies on interference between an unscattered reference wave and a weakly scattered object wave - an assumption that rapidly fails in turbid samples under multiple scattering. To overcome this…

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Fourier ptychographic microscopy (FPM) is a promising quantitative phase imaging technique that enables high-resolution, label-free imaging over a large field-of-view. Here, we present the first application of FPM for the quantitative…

We present a widefield two-dimensional electronic spectroscopy microscope (2DESM) that integrates multidimensional coherent spectroscopy with optical imaging, enabling femtosecond temporal and micrometer spatial resolution. The broadband…

We present Defocusing Microscopy (DM), a bright-field optical microscopy technique able to perform total 3D imaging of transparent objects. By total 3D imaging we mean the determination of the actual shapes of the upper and lower surfaces…

Biological Physics · Physics 2015-06-19 P. M. S. Roma , L. Siman , F. T. Amaral , U. Agero , O. N. Mesquita

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at 24kHz while keeping the lateral resolution gain and background rejection of a regular label-free ISM setup. Our method uses a…

Optics · Physics 2023-08-31 Duc-Minh Ta , Alberto Aguilar , Pierre Bon

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

Microsphere-based subwavelength imaging technique was first demonstrated in 2011. After nearly a decade of efforts, such technique has spawned numerous interests in fields such as laser nano-machining, imaging, sensing and biological…

Optics · Physics 2021-08-26 Bing Yan , Yang Song , Xibin Yang , Daxi Xiong , Zengbo Wang

Conventional microscope objective lenses are diffraction limited, which means that they cannot resolve features smaller than half the illumination wavelength. Under white light illumination, such resolution limit is about 250-300 nm for an…

Instrumentation and Detectors · Physics 2021-08-26 Bing Yan , Zengbo Wang , Alan Parker , Yukun Lai , John Thomas , Liyang Yue , James Monks

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method…

Optics · Physics 2025-09-11 Charlie Kersuzan , Anirban Jana , Amaury Badon

Spatial resolution in optical microscopy has traditionally been treated as a fixed parameter of the optical system. Here, we present an approach to enhance transverse resolution in beam-scanned optical coherence tomography (OCT) beyond its…

Optics · Physics 2021-10-22 Nichaluk Leartprapun , Steven G. Adie

Aberration-corrected scanning transmission electron microscopes (STEM) provide sub-angstrom lateral resolution; however, the large convergence angle greatly reduces the depth of field. For microscopes with a small depth of field,…

Materials Science · Physics 2011-12-15 Robert Hovden , Huolin L. Xin , David A. Muller