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Tracking living cells in video sequence is difficult, because of cell morphology and high similarities between cells. Tracking-by-detection methods are widely used in multi-cell tracking. We perform multi-cell tracking based on the cell…

Computer Vision and Pattern Recognition · Computer Science 2019-06-27 Zibin Zhou , Fei Wang , Wenjuan Xi , Huaying Chen , Peng Gao , Chengkang He

Cell tracking remains a pivotal yet challenging task in biomedical research. The full potential of deep learning for this purpose is often untapped due to the limited availability of comprehensive and varied training data sets. In this…

Computer Vision and Pattern Recognition · Computer Science 2024-04-30 Moritz Sturm , Lorenzo Cerrone , Fred A. Hamprecht

An automatic approach to counting any kind of cells could alleviate work of the experts and boost the research in fields such as regenerative medicine. In this paper, a method for microscopy cell counting using multiple frames (hence…

Computer Vision and Pattern Recognition · Computer Science 2018-02-01 Alexander Gomez Villa , Augusto Salazar , Igor Stefanini

Semantic segmentation of microscopic cell images using deep learning is an important technique, however, it requires a large number of images and ground truth labels for training. To address the above problem, we consider an efficient…

Computer Vision and Pattern Recognition · Computer Science 2023-04-18 Sota Kato , Kazuhiro Hotta

Tracking cells and detecting mitotic events in time-lapse microscopy image sequences is a crucial task in biomedical research. However, it remains highly challenging due to dividing objects, low signal-tonoise ratios, indistinct boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2025-09-15 Zhu Chen , Mert Edgü , Er Jin , Johannes Stegmaier

We propose a general framework for a collaborative machine learning system to assist bioscience researchers with the task of labeling specific cell identities from microscopic still or video imaging. The distinguishing features of this…

Quantitative Methods · Quantitative Biology 2019-03-25 Greg Bubnis , Steven Ban , Matthew D. DiFranco , Saul Kato

We propose a 3D convolutional neural network to simultaneously segment and detect cell nuclei in confocal microscopy images. Mirroring the co-dependency of these tasks, our proposed model consists of two serial components: the first part…

Image and Video Processing · Electrical Eng. & Systems 2018-09-07 Sundaresh Ram , Vicky T. Nguyen , Kirsten H. Limesand , Mert R. Sabuncu

We present a method for microtubule tracking in electron microscopy volumes. Our method first identifies a sparse set of voxels that likely belong to microtubules. Similar to prior work, we then enumerate potential edges between these…

Computer Vision and Pattern Recognition · Computer Science 2020-09-18 Nils Eckstein , Julia Buhmann , Matthew Cook , Jan Funke

Cell counting in microscopy images is vital in medicine and biology but extremely tedious and time-consuming to perform manually. While automated methods have advanced in recent years, state-of-the-art approaches tend to increasingly…

Computer Vision and Pattern Recognition · Computer Science 2025-03-19 Zixuan Zheng , Yilei Shi , Chunlei Li , Jingliang Hu , Xiao Xiang Zhu , Lichao Mou

The nematode Caenorhabditis elegans (C. elegans) is used as a model organism to better understand developmental biology and neurobiology. C. elegans features an invariant cell lineage, which has been catalogued and observed using…

Computer Vision and Pattern Recognition · Computer Science 2022-08-03 Andrew Lauziere , Ryan Christensen , Hari Shroff

A human watching a video of closely-packed cells can generally identify every individual cell, regardless of density and noise, but most currently-available cell-tracking software cannot. This is because the human brain automatically builds…

Cell Behavior · Quantitative Biology 2017-09-27 Huy Pham , Emile Ramez Shehada , Shawna Stahlheber , Wayne B. Hayes

Determining cell identities in imaging sequences is an important yet challenging task. The conventional method for cell identification is via cell tracking, which is complex and can be time-consuming. In this study, we propose an innovative…

Quantitative Methods · Quantitative Biology 2024-03-05 Baiyang Dai , Jiamin Yang , Hari Shroff , Patrick La Riviere

Cell shape analysis is important in biomedical research. Deep learning methods may perform to segment individual cells if they use sufficient training data that the boundary of each cell is annotated. However, it is very time-consuming for…

Image and Video Processing · Electrical Eng. & Systems 2020-02-26 Kazuya Nishimura , Dai Fei Elmer Ker , Ryoma Bise

Particle tracking is a powerful biophysical tool that requires conversion of large video files into position time series, i.e. traces of the species of interest for data analysis. Current tracking methods, based on a limited set of input…

Quantitative Methods · Quantitative Biology 2018-10-09 Jay M. Newby , Alison M. Schaefer , Phoebe T. Lee , M. Gregory Forest , Samuel K. Lai

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

We propose a novel weakly supervised method to improve the boundary of the 3D segmented nuclei utilizing an over-segmented image. This is motivated by the observation that current state-of-the-art deep learning methods do not result in…

Computer Vision and Pattern Recognition · Computer Science 2021-08-31 S. Shailja , Jiaxiang Jiang , B. S. Manjunath

Signaling pathways are responsible for the regulation of cell processes, such as monitoring the external environment, transmitting information across membranes, and making cell fate decisions. Given the increasing amount of biological data…

Molecular Networks · Quantitative Biology 2020-04-07 Daniel Inostroza , Cecilia Hernández , Diego Seco , Gonzalo Navarro , Alvaro Olivera-Nappa

The ability to quickly and accurately identify microbial species in a sample, known as metagenomic profiling, is critical across various fields, from healthcare to environmental science. This paper introduces a novel method to profile…

Genomics · Quantitative Biology 2025-04-10 Riselda Kodra , Hadjer Benmeziane , Irem Boybat , William Andrew Simon

Representation learning has driven major advances in natural image analysis by enabling models to acquire high-level semantic features. In microscopy imaging, however, it remains unclear what current representation learning methods actually…

Computer Vision and Pattern Recognition · Computer Science 2026-03-17 Ivan Svatko , Maxime Sanchez , Ihab Bendidi , Gilles Cottrell , Auguste Genovesio

High-throughput screening techniques, such as microscopy imaging of cellular responses to genetic and chemical perturbations, play a crucial role in drug discovery and biomedical research. However, robust perturbation screening for…

Computer Vision and Pattern Recognition · Computer Science 2025-07-16 Jiayuan Chen , Thai-Hoang Pham , Yuanlong Wang , Ping Zhang