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Cell tracking and segmentation assist biologists in extracting insights from large-scale microscopy time-lapse data. Driven by local accuracy metrics, current tracking approaches often suffer from a lack of long-term consistency and the…

Computer Vision and Pattern Recognition · Computer Science 2025-06-30 Timo Kaiser , Maximilian Schier , Bodo Rosenhahn

This paper presents a method for time-lapse 3D cell analysis. Specifically, we consider the problem of accurately localizing and quantitatively analyzing sub-cellular features, and for tracking individual cells from time-lapse 3D confocal…

Computer Vision and Pattern Recognition · Computer Science 2022-08-18 Jiaxiang Jiang , Amil Khan , S. Shailja , Samuel A. Belteton , Michael Goebel , Daniel B. Szymanski , B. S. Manjunath

Automatic detection and tracking of cells in microscopy images are major applications of computer vision technologies in both biomedical research and clinical practice. Though machine learning methods are increasingly common in these…

Computer Vision and Pattern Recognition · Computer Science 2023-12-07 Nikomidisz Eftimiu , Michal Kozubek

With the growth of artificial intelligence (AI), there has been an increase in the adoption of computer vision and deep learning (DL) techniques for the evaluation of microscopy images and movies. This adoption has not only addressed…

Quantitative Methods · Quantitative Biology 2023-07-21 Binghao Chai , Christoforos Efstathiou , Haoran Yue , Viji M. Draviam

High-throughput screening using cell images is an efficient method for screening new candidates for pharmaceutical drugs. To complete the screening process, it is essential to have an efficient process for analyzing cell images. This paper…

Computer Vision and Pattern Recognition · Computer Science 2024-02-28 Mizuki Fukasawa , Tomokazu Fukuda , Takuya Akashi

Combining experiments with artificial intelligence algorithms, we propose a new machine learning based approach to extract the cellular force distributions from the microscope images. The full process can be divided into three steps. First,…

Cell tracking algorithms which automate and systematise the analysis of time lapse image data sets of cells are an indispensable tool in the modelling and understanding of cellular phenomena. In this study we present a theoretical framework…

We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

The automated segmentation and tracking of macrophages during their migration are challenging tasks due to their dynamically changing shapes and motions. This paper proposes a new algorithm to achieve automatic cell tracking in time-lapse…

Image and Video Processing · Electrical Eng. & Systems 2023-01-03 Seol Ah Park , Tamara Sipka , Zuzana Kriva , George Lutfalla , Mai Nguyen-Chi , Karol Mikula

The study of mouse social behaviours has been increasingly undertaken in neuroscience research. However, automated quantification of mouse behaviours from the videos of interacting mice is still a challenging problem, where object tracking…

Computer Vision and Pattern Recognition · Computer Science 2022-03-28 Zheheng Jiang , Zhihua Liu , Long Chen , Lei Tong , Xiangrong Zhang , Xiangyuan Lan , Danny Crookes , Ming-Hsuan Yang , Huiyu Zhou

We propose a novel approach to automatically tracking cell populations in time-lapse images. To account for cell occlusions and overlaps, we introduce a robust method that generates an over-complete set of competing detection hypotheses. We…

Computer Vision and Pattern Recognition · Computer Science 2016-01-27 Engin Türetken , Xinchao Wang , Carlos Becker , Carsten Haubold , Pascal Fua

Video microscopy has a long history of providing insights and breakthroughs for a broad range of disciplines, from physics to biology. Image analysis to extract quantitative information from video microscopy data has traditionally relied on…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Benjamin Midtvedt , Saga Helgadottir , Aykut Argun , Jesús Pineda , Daniel Midtvedt , Giovanni Volpe

Tracking all nuclei of an embryo in noisy and dense fluorescence microscopy data is a challenging task. We build upon a recent method for nuclei tracking that combines weakly-supervised learning from a small set of nuclei center point…

Computer Vision and Pattern Recognition · Computer Science 2022-08-25 Peter Hirsch , Caroline Malin-Mayor , Anthony Santella , Stephan Preibisch , Dagmar Kainmueller , Jan Funke

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

State-of-the-art object detection and segmentation methods for microscopy images rely on supervised machine learning, which requires laborious manual annotation of training data. Here we present a self-supervised method based on time arrow…

Computer Vision and Pattern Recognition · Computer Science 2023-07-27 Benjamin Gallusser , Max Stieber , Martin Weigert

Object tracking is a fundamental tool in modern innovation, with applications in defense systems, autonomous vehicles, and biomedical research. It enables precise identification, monitoring, and spatiotemporal analysis of objects across…

Computer Vision and Pattern Recognition · Computer Science 2024-12-03 Mojtaba S. Fazli , Shannon Quinn

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

Conventional cell tracking methods detect multiple cells in each frame (detection) and then associate the detection results in successive time-frames (association). Most cell tracking methods perform the association task independently from…

Computer Vision and Pattern Recognition · Computer Science 2020-02-27 Junya Hayashida , Kazuya Nishimura , Ryoma Bise

Quantitative analysis of the dynamics of tiny cellular and sub-cellular structures, known as particles, in time-lapse cell microscopy sequences requires the development of a reliable multi-target tracking method capable of tracking numerous…

Computer Vision and Pattern Recognition · Computer Science 2015-07-24 Seyed Hamid Rezatofighi , Stephen Gould , Ba Tuong Vo , Ba-Ngu Vo , Katarina Mele , Richard Hartley

The classification of microscopy videos capturing complex cellular behaviors is crucial for understanding and quantifying the dynamics of biological processes over time. However, it remains a frontier in computer vision, requiring…