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Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D…

In this study, a method to construct a full-colour volumetric display is presented using a commercially available inkjet printer. Photoreactive luminescence materials are minutely and automatically printed as the volume elements, and…

Imaging with optical resolution through and inside complex samples is a difficult challenge with important applications in many fields. The fundamental problem is that inhomogeneous samples, such as biological tissues, randomly scatter and…

Optics · Physics 2014-10-17 Ori Katz , Pierre Heidmann , Mathias Fink , Sylvain Gigan

Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…

Corneal evaluation in ophthalmology necessitates cellular-resolution and fast imaging techniques allowing accurate diagnoses. Currently, the fastest volumetric imaging technique is Fourier-domain full-field optical coherence tomography…

Three-dimensional imaging of biological cells is crucial for the investigation of cell biology, provide valuable information to reveal the mechanisms behind pathophysiology of cells and tissues. Recent advances in optical diffraction…

Biological Physics · Physics 2016-03-03 Kyoohyun Kim , Jonghee Yoon , Seungwoo Shin , SangYun Lee , Su-A Yang , YongKeun Park

Correlation of neuropathology with MRI has the potential to transfer microscopic signatures of pathology to in vivo scans. There is increasing interest in building these correlations from 3D reconstructed stacks of slab photographs, which…

Label-free characterization of biological specimens seeks to supplement existing imaging techniques and avoid the need for contrast agents that can disturb the native state of living samples. Conventional label-free optical imaging…

We propose a method to directly visualize the photonic band-structure of micron size photonic crystals using wide angle spectroscopy. By extending Fourier Imaging Spectroscopy sensitivity into the infrared range we have obtained accurate…

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful…

Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-31 Sourabh Bhide , Ralf Mikut , Maria Leptin , Johannes Stegmaier

Conventionally, 3-dimensional cellular tomography can be done with light sheet or multi-angle observations. Recently, a new technique was introduced where the cell was rotated using convection currents to visualize the outer periphery (Liu…

X-Ray imaging is quick, cheap and useful for front-line care assessment and intra-operative real-time imaging (e.g., C-Arm Fluoroscopy). However, it suffers from projective information loss and lacks vital volumetric information on which…

Image and Video Processing · Electrical Eng. & Systems 2020-06-24 Athanasios Vlontzos , Samuel Budd , Benjamin Hou , Daniel Rueckert , Bernhard Kainz

Lensless X-ray imaging provides element-specific nanoscale insights into thick samples beyond the reach of conventional light and electron microscopy. Coherent diffraction imaging (CDI) methods, such as ptychographic tomography, can recover…

Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…

The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and…

The goal of 3D visualization is to provide the user with an intuitive interface which enables him to explore the 3D data in an interactive manner. The aim of the exploration is to identify and analyze anomalies or to give proof of the…

Graphics · Computer Science 2018-12-12 Pratik Kalshetti , Parag Rahangdale , Dinesh Jangra , Manas Bundele , Chiranjoy Chattopadhyay

New lensless diffractive X-ray technic for micro-scale imaging of biological tissue is based on quantitative phase retrieval schemes. By incorporating refraction, this method yields improved contrast compared to purely absorption-based…

Numerical Analysis · Mathematics 2018-03-28 Victor Palamodov

Three-dimensional (3D) subcellular imaging is essential for biomedical research, but the diffraction limit of optical microscopy compromises axial resolution, hindering accurate 3D structural analysis. This challenge is particularly…