Interface Self-Referenced Dynamic Full-Field Optical Coherence Tomography
Abstract
Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D samples. However, D-FFOCT suffers from fringe artefacts when imaging nearby reflective surfaces and is highly sensitive to vibrations. Here, we present interface Self-Referenced (iSR) D-FFOCT, an alternative configuration to D-FFOCT that takes advantage of the presence of the sample coverslip in between the sample and the objective by using it as a defocused reference arm, thus avoiding the aforementioned artefacts. We demonstrate the ability of iSR D-FFOCT to image 2D fibroblast cell cultures, which are among the flattest mammalian cells.
Cite
@article{arxiv.2302.08839,
title = {Interface Self-Referenced Dynamic Full-Field Optical Coherence Tomography},
author = {Tual Monfort and Salvatore Azzollini and Tasnim Ben Yacoub and Isabelle Audo and Sacha Reichman and Kate Grieve and Olivier Thouvenin},
journal= {arXiv preprint arXiv:2302.08839},
year = {2023}
}