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Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Integrated photonic devices have become pivotal elements across most research fields that involve light-based applications. A particularly versatile category of this technology are programmable photonic integrated processors, which are…

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

We introduce a phase imaging mechanism for scanning transmission electron microscopy that exploits the complementary intensity changes of transmitted disks at different scattering angles. For scanning transmission electron microscopy, this…

Instrumentation and Detectors · Physics 2023-07-06 Binbin Wang , David W. McComb

Stimulated Emission Depletion (STED) microscopy has emerged as a powerful technique providing visualization of biological structures at the molecular level in living samples. In this technique, the diffraction limit is broken by selectively…

We propose a novel stimulated emission depletion (STED) microscopy based on array detection and photon reassignment. By replacing the single-point detector in traditional STED with a detector array and utilizing the photon reassignment…

Single Pixel (SP) imaging is now a reality in many applications, e.g., biomedical ultrathin endoscope and fluorescent spectroscopy. In this context, many schemes exist to improve the light throughput of these device, e.g., using structured…

Computer Vision and Pattern Recognition · Computer Science 2019-02-19 Amirafshar Moshtaghpour , José M. Bioucas-Dias , Laurent Jacques

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction…

Accessing the point-spread function (PSF) of a complex optical system is important for a variety of imaging applications. However, placing an invasive point source is often impractical, and estimating it blindly with multiple frames is slow…

Optics · Physics 2020-09-23 Tengfei Wu , Jonathan Dong , Sylvain Gigan

Structured light 3D surface imaging is a school of techniques in which structured light patterns are used for measuring the depth map of the object. Among all the designed structured light patterns, phase pattern has become most popular…

Image and Video Processing · Electrical Eng. & Systems 2020-09-14 Zhenzhou Wang

We present a novel approach to implement compressive sensing in laser scanning microscopes (LSM), specifically in image scanning microscopy (ISM), using a single-photon avalanche diode (SPAD) array detector. Our method addresses two…

Image and Video Processing · Electrical Eng. & Systems 2023-07-20 Ajay Gunalan , Marco Castello , Simonluca Piazza , Shunlei Li , Alberto Diaspro , Leonardo S. Mattos , Paolo Bianchini

Accurate blur estimation is essential for high-performance imaging across various applications. Blur is typically represented by the point spread function (PSF). In this paper, we propose a physics-informed PSF learning framework for…

Computer Vision and Pattern Recognition · Computer Science 2025-03-12 Liqun Chen , Yuxuan Li , Jun Dai , Jinwei Gu , Tianfan Xue

The wavefront is scrambled when coherent light propagates through a random scattering medium and which makes direct use of the conventional optical methods ineffective. In this paper, we propose and demonstrate a structured light…

Localization microscopy enables imaging with resolutions that surpass the conventional optical diffraction limit. Notably, the MINFLUX method achieves super-resolution by shaping the excitation point-spread function (PSF) to minimize the…

A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and…

Optics · Physics 2009-08-26 P. Ferrand , M. Pianta , A. Kress , A. Aillaud , H. Rigneault , D. Marguet

Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…

Optics · Physics 2020-11-11 Xianye Li , Yafei sun , Yikang He , Xun Li , Baoqing Sun

Over the past decade, structured illumination microscopy (SIM) has found its niche in super-resolution (SR) microscopy due to its fast imaging speed and low excitation intensity. However, due to the significantly higher light dose compared…

Point Spread Function (PSF) modeling is a central part of any astronomy data analysis relying on measuring the shapes of objects. It is especially crucial for weak gravitational lensing, in order to beat down systematics and allow one to…

Instrumentation and Methods for Astrophysics · Physics 2015-05-30 Joel Bergé , Sedona Price , Adam Amara , Jason Rhodes

Scanning transmission electron microscopy (STEM) has been extensively used for imaging complex materials down to atomic resolution. The most commonly employed STEM modality, annular dark-field imaging, produces easily-interpretable…

Continuous wavefront sensing benefits space observatories in on-orbit optical performance maintenance. To measure the phase of a wavefront, phase retrieval is an attractive technique as it uses multiple point spread function (PSF) images…

Instrumentation and Methods for Astrophysics · Physics 2024-09-17 Hyukmo Kang , Kyle Van Gorkom , Meghdoot Biswas , Daewook Kim , Ewan S. Douglas