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Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

Optics · Physics 2024-10-16 Doron Shterman , Guy Bartal

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically…

Optics · Physics 2017-05-11 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

A point spread function (PSF) describes the distribution of light for a pure point source in an astronomical image due to the optics of the instrument. An accurate PSF is key for deconvolution, point source photometry and source removal.…

Instrumentation and Methods for Astrophysics · Physics 2025-07-28 Ava Polzin

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…

Biological Physics · Physics 2018-08-10 Chiara Garbellotto , Jonathan M. Taylor

The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

Data Analysis, Statistics and Probability · Physics 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

Recently introduced angular-memory-effect based techniques enable non-invasive imaging of objects hidden behind thin scattering layers. However, both the speckle-correlation and the bispectrum analysis are based on the statistical average…

Optics · Physics 2017-11-22 Tengfei Wu , Jonathan Dong , Xiaopeng Shao , Sylvain Gigan

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…

Image and Video Processing · Electrical Eng. & Systems 2026-03-19 Jiaming Qian , Jing Feng , Hongjun Wu , Maoxian Zhang , Dongqin Lu , Tianchi Kang , Xinyu Han , Qian Chen , Chao Zuo

Inspired by the capability of structured illumination microscopy in subwavelength imaging, many researchers devoted themselves to investigating this methodology. However, due to the free propagating feature of the traditional structured…

We present projective parallel single-pixel imaging (pPSI), a 3D photography method that provides a robust and efficient way to analyze the light transport behavior and enables separation of light effect due to global illumination, thereby…

Image and Video Processing · Electrical Eng. & Systems 2023-12-14 Yuxi Li , Hongzhi Jiang , Huijie Zhao , Xudong Li

We present super-resolved coherent anti-Stokes Raman scattering (CARS) microscopy by implementing phase-resolved image scanning microscopy (ISM), achieving up to two-fold resolution increase as compared with a conventional CARS microscope.…

Optics · Physics 2024-11-22 Anna Zhitnitsky , Elad Benjamin , Ora Bitton , Dan Oron

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

Optics · Physics 2022-03-09 James D. Manton

Three-dimensional spatiotemporal tracking of microscopic particles in multiple colors is a challenging optical imaging task. Existing approaches require a trade-off between photon-efficiency, field of view, mechanical complexity, spectral…

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Here we apply hyperspectral bright field imaging to collect computed tomographic images with excellent energy resolution (800 eV), applying it for the first time to map the distribution of stain in a fixed biological sample through its…

Point-spread-function (PSF) engineering is a powerful computational imaging techniques wherein a custom phase mask is integrated into an optical system to encode additional information into captured images. Used in combination with deep…

Computer Vision and Pattern Recognition · Computer Science 2023-03-31 Sachin Shah , Sakshum Kulshrestha , Christopher A. Metzler

Structured illumination microscopy (SIM) can double the resolution beyond the light diffraction limit, but it comes at the cost of multiple camera exposures and the heavy computation burden of multiple Fourier transforms. In this paper, we…

Image and Video Processing · Electrical Eng. & Systems 2021-07-14 Qinnan Zhang , En Bo , Jiawei Chen , Jiaosheng Li , Heming Jiang , Xiaoxu Lu , Liyun Zhong , Jindong Tian

Reconstruction of the point spread function (PSF) plays an important role in many areas of astronomy, including photometry, astrometry, galaxy morphology, and shear measurement. The atmospheric and instrumental effects are the two main…

Instrumentation and Methods for Astrophysics · Physics 2024-04-25 Pedro Alonso , Jun Zhang , Cong Liu