Super-resolved CARS by coherent image scanning
Abstract
We present super-resolved coherent anti-Stokes Raman scattering (CARS) microscopy by implementing phase-resolved image scanning microscopy (ISM), achieving up to two-fold resolution increase as compared with a conventional CARS microscope. Phase-sensitivity is required for the standard pixel-reassignment procedure since the scattered field is coherent, thus the point-spread function (PSF) is well-defined only for the field amplitude. We resolve the complex field by a simple add-on to the CARS setup enabling inline interferometry. Phase-sensitivity offers additional contrast which informs the spatial distribution of both resonant and nonresonant scatterers. As compared with alternative super-resolution schemes in coherent nonlinear microscopy, the proposed method is simple, requires only low-intensity excitation, and is compatible with any conventional forward-detected CARS imaging setup.
Cite
@article{arxiv.2404.15094,
title = {Super-resolved CARS by coherent image scanning},
author = {Anna Zhitnitsky and Elad Benjamin and Ora Bitton and Dan Oron},
journal= {arXiv preprint arXiv:2404.15094},
year = {2024}
}